Piperacillin, clavulanic acid, and tazobactam were purchased from Sigma-Aldrich. Avibactam was obtained from Advanced Chemblocks. Enmetazobactam, vaborbactam, and relebactam were acquired from MedChemExpress.
Vaborbactam
Manufactured by MedChemExpress
Vaborbactam is a laboratory product manufactured by MedChemExpress. It functions as a beta-lactamase inhibitor, designed to restore the efficacy of certain antibiotics against resistant bacterial strains.
Automatically generated - may contain errors
Lab products found in correlation
Relebactam, by MedChemExpress (2 mentions)
Piperacillin, by Merck Group (1 mentions)
Tazobactam, by Merck Group (1 mentions)
Clavulanic acid, by Merck Group (1 mentions)
Avibactam, by Abbvie (1 mentions)
Plasmid purification kit, by Qiagen (1 mentions)
Growth media, by Thermo Fisher Scientific (1 mentions)
Avibactam, by MedChemExpress (1 mentions)
3 protocols using vaborbactam
1
Antimicrobial Combination Evaluation
2
Antimicrobial Susceptibility Testing Protocol
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Antimicrobial susceptibility testing was performed by reference broth microdilution methods conducted according to Clinical and Laboratory Standards Institute (CLSI) procedures [14 ]. Quality control testing was performed daily to ensure proper test conditions. Quality control strains included E coli ATCC 25922 and NCTC 13353, K pneumoniae ATCC 700603, ATCC BAA-1705 and BAA-2814, and Pseudomonas aeruginosa ATCC 27853. CLSI guidelines were used for the interpretation of susceptibility rates, with the exception of tigecycline, for which US Food and Drug Administration (FDA) breakpoints were applied [15 , 16 ]. Avibactam was provided by Allergan. Other agents were acquired from Sigma-Aldrich (St Louis, Missouri), US Pharmacopeia (Rockville, Maryland), Advanced Chemblocks (Hayward, California; relebactam), or MedChemExpress (Monmouth Junction, New Jersey; vaborbactam).
3
Carbapenemase-producing Klebsiella Resistance Mechanism
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Chemicals were from Sigma and growth media from Oxoid, unless otherwise stated.
Meropenem was from Sequoia Research Products, vaborbactam and avibactam were from MedChemExpress. Strains used were K. pneumoniae Ecl8 (31) the TEM-1-producing ramR mutant (Arg44FS) clinical isolate KP21, the wild-type clinical isolate KP47 and the in vitroselected Ecl8-derived oqxR (Tyr109STOP) or ramR (Thr124Pro) loss-of-function mutants (29) . A plasmid carrying bla OXA-232 (pOXA-232) was recovered from K. pneumoniae clinical isolate KP11 (29) using a Qiagen plasmid purification kit, with the plasmid then used to transform E. coli DH5α to reduced piperacillin/tazobactam susceptibility (8 µg.mL -1 piperacillin and 4 µg.mL -1 tazobactam) using electroporation. Recombinants were confirmed to be cefotaxime susceptible before the plasmid was re-purified and used to transform K.
pneumoniae isolates. The bla OXA-232 -encoding region was confirmed as being unchanged from the original using PCR sequencing.
Meropenem was from Sequoia Research Products, vaborbactam and avibactam were from MedChemExpress. Strains used were K. pneumoniae Ecl8 (31) the TEM-1-producing ramR mutant (Arg44FS) clinical isolate KP21, the wild-type clinical isolate KP47 and the in vitroselected Ecl8-derived oqxR (Tyr109STOP) or ramR (Thr124Pro) loss-of-function mutants (29) . A plasmid carrying bla OXA-232 (pOXA-232) was recovered from K. pneumoniae clinical isolate KP11 (29) using a Qiagen plasmid purification kit, with the plasmid then used to transform E. coli DH5α to reduced piperacillin/tazobactam susceptibility (8 µg.mL -1 piperacillin and 4 µg.mL -1 tazobactam) using electroporation. Recombinants were confirmed to be cefotaxime susceptible before the plasmid was re-purified and used to transform K.
pneumoniae isolates. The bla OXA-232 -encoding region was confirmed as being unchanged from the original using PCR sequencing.
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