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2 protocols using puromycin dihydrochloride

1

Reagents for Immune Stimulation

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Escherichia coli 0111:B4 lipopolysaccharide (LPS), zymosan from Saccharomyces cerevisiae, Z-Leu-Leu-Leu-al (MG-132), and D-desthiobiotin were purchased from Sigma-Aldrich (St. Louis, MO, USA). CpG oligodeoxynucleotides were purchased from Invivogen (Toulouse, France). N-Acetyl-Leu-Leu-Norleu-al (ALLN) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Puromycin dihydrochloride was purchased from Gold Biotechnology (St. Louis, MO, USA).
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2

Quantifying Cell Cytoskeletal and Signaling Dynamics

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Chemical reagents used were as follows: Alexa Fluor 647 phalloidin (Cat# A22287; Thermo Fisher), Alexa Fluor plus 405 phalloidin (Cat# A30104; Thermo Fisher), D-erythro-sphingosine-1-phosphate (Cat# 860492; Avanti Polar Lipids), normal donkey serum (Cat# 017–000-121; Jackson Immunoresearch), rapamycin (Cat# R5000; LC Laboratories), puromycin dihydrochloride (Cat# P-600-100; Gold Biotechnology), insulin (Cat# I-1882; Sigma-Aldrich), human holo transferrin (Cat# T-4132; Sigma-Aldrich), sodium selenite (Cat# 5–5261; Sigma-Aldrich), FA-free BSA (Cat# A8806; Sigma-Aldrich), oleic acid (Cat# O7501; Sigma-Aldrich), collagen I, bovine (Cat# A10644-01; GIBCO BRL), Alexa Fluor 405 NHS Ester (succinimidyl ester; Cat# A30000; Thermo Fisher), Oregon Green-488–conjugated gelatin (Cat# G13186; Thermo Fisher), ascorbic acid (Cat# A4034; Sigma-Aldrich), and basic fibroblast growth factor (Cat# 234-FSE; R&D Systems), VEGF165, human (Cat# 1150–05-10; Goldbiotech), GM6001 MMP Inhibitor (Cat# CC1000; Sigma-Aldrich), MT1-MMP inhibitor, NSC405020 (Cat# 444295; Sigma-Aldrich), blebbistatin (Cat# 2406–1; BioVision), and saracatinib (Cat# sc-45364607; Santa Cruz).
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