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S 5 adenosyl l methionine iodide sam

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S-(5′-Adenosyl)-L-Methionine Iodide (SAM) is a chemical compound used as a laboratory reagent. It is a co-factor involved in various biochemical reactions and is commonly used in research applications.

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Lab products found in correlation

Dasatinib, by Selleck Chemicals (3 mentions) Pf 573228, by Selleck Chemicals (3 mentions) Jph203, by Selleck Chemicals (3 mentions) Fibronectin, by Merck Group (3 mentions) N acetyl l cysteine nac, by Merck Group (3 mentions) Nct 503, by Merck Group (3 mentions) Latrunculin a, by Cayman Chemical (3 mentions) Dntps, by Euroclone (3 mentions) Pcw57 gfp p2a mcs, by Addgene (3 mentions) Cytochalasin d, by Merck Group (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) 17 aag geldanamycin, by LC Laboratories (3 mentions) Anti adma antibody, by Cell Signaling Technology (1 mentions)

4 protocols using s 5 adenosyl l methionine iodide sam

1

Ectopic Expression of TP53 Variants

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The following compounds and working concentrations were used: Fibronectin (10 μg/ml, Sigma Aldrich F0895), JPH203 (Selleck Chemicals S8667), NCT503 (Sigma Aldrich SML1659), Dasatinib (Selleck Chemicals S1021), PF-573228 (Selleck Chemicals S2013), Cytochalasin D (Sigma Aldrich C2618), Latrunculin-A (Cayman 10010630), SAHA Cayman 149647-78-9), 17-AAG Geldanamycin (Lc Laboratories A-6880), GCN2-IN-1 (Synonyms: A-92; MedChem Express Cat. No.: HY-100877), nucleosides dNTPs (Euroclone EMR276425), N-Acetyl-L-Cysteine (NAC) (Sigma Aldrich A9165), S-(5′-Adenosyl)-L-Methionine Iodide (SAM) (Sigma Aldrich A4377) and DMSO (Sigma Aldrich D4540). Treatments lasted as described in figure legends.
pCW57-GFP-P2A-MCS (empty backbone) was bought from Addgene (plasmid #89181). pCW57-GFP-HA-p53 wt, pCW57-GFP-HA-mutp53 R175H, pCW57-GFP-HA-mutp53 R249S, pCW57-GFP-HA-mutp53 R273K or pCW57-GFP-HA-mutp53 R280K were generated by cloning a PCR amplified DNA fragment of the human HA-tagged TP53 sequence (WT or mutated) into the pCW57-GFP-P2A-MCS with MluI and BamHI restriction sites.
Tombari C., Zannini A., Bertolio R., Pedretti S., Audano M., Triboli L., Cancila V., Vacca D., Caputo M., Donzelli S., Segatto I., Vodret S., Piazza S., Rustighi A., Mantovani F., Belletti B., Baldassarre G., Blandino G., Tripodo C., Bicciato S., Mitro N, & Del Sal G. (2023). Mutant p53 sustains serine-glycine synthesis and essential amino acids intake promoting breast cancer growth. Nature Communications, 14, 6777.
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2

In Vivo and In Vitro Arginine Methylation Assays

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For in vivo arginine methylation, HEK293T cells were co-transfected with GST-6PGD/GST-ENO1/GST-aldolase A (ALDOA) and Flag-PRMT6 plasmids. Thirty-six hours later, the cells were lysed with NP40 lysis buffer and IP using an anti-GST antibody was performed to pull down the GST-6PGD/GST-ENO1/GST-ALDOA protein. The input and eluted protein lysates were resolved by SDS-PAGE and analyzed by Western blotting with indicated antibodies. The level of asymmetric dimethylated arginine (ADMA) in GST-6PGD/GST-ENO1/GST-ALDOA was assessed by using an anti-ADMA antibody (Cell signaling, MA, USA).
For in vitro arginine methylation assay, recombination Flag-PRMT6 (or Flag-PRMT6 purified from HEK293T cells) and recombination GST-6PGD/recombination GST-ENO1 (rGST-6PGD/rGST-ENO1) proteins were incubated in methylation rea-ction buffer (50 mmol/L Tris, 150 mmol/L NaCl), with 16 μmol/L methyl donor S-(5′-adenosyl)-l-methionine iodide (SAM, sigma, MO, USA), to a total volume of 60 μL and incubated at 30 °C for 120 min.
Sun M., Li L., Niu Y., Wang Y., Yan Q., Xie F., Qiao Y., Song J., Sun H., Li Z., Lai S., Chang H., Zhang H., Wang J., Yang C., Zhao H., Tan J., Li Y., Liu S., Lu B., Liu M., Kong G., Zhao Y., Zhang C., Lin S.H., Luo C., Zhang S, & Shan C. (2022). PRMT6 promotes tumorigenicity and cisplatin response of lung cancer through triggering 6PGD/ENO1 mediated cell metabolism. Acta Pharmaceutica Sinica. B, 13(1), 157-173.
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3

Ectopic Expression of TP53 Variants

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The following compounds and working concentrations were used: Fibronectin (10 μg/ml, Sigma Aldrich F0895), JPH203 (Selleck Chemicals S8667), NCT503 (Sigma Aldrich SML1659), Dasatinib (Selleck Chemicals S1021), PF-573228 (Selleck Chemicals S2013), Cytochalasin D (Sigma Aldrich C2618), Latrunculin-A (Cayman 10010630), SAHA Cayman 149647-78-9), 17-AAG Geldanamycin (Lc Laboratories A-6880), GCN2-IN-1 (Synonyms: A-92; MedChem Express Cat. No.: HY-100877), nucleosides dNTPs (Euroclone EMR276425), N-Acetyl-L-Cysteine (NAC) (Sigma Aldrich A9165), S-(5′-Adenosyl)-L-Methionine Iodide (SAM) (Sigma Aldrich A4377) and DMSO (Sigma Aldrich D4540). Treatments lasted as described in figure legends.
pCW57-GFP-P2A-MCS (empty backbone) was bought from Addgene (plasmid #89181). pCW57-GFP-HA-p53 wt, pCW57-GFP-HA-mutp53 R175H, pCW57-GFP-HA-mutp53 R249S, pCW57-GFP-HA-mutp53 R273K or pCW57-GFP-HA-mutp53 R280K were generated by cloning a PCR amplified DNA fragment of the human HA-tagged TP53 sequence (WT or mutated) into the pCW57-GFP-P2A-MCS with MluI and BamHI restriction sites.
Tombari C., Zannini A., Bertolio R., Pedretti S., Audano M., Triboli L., Cancila V., Vacca D., Caputo M., Donzelli S., Segatto I., Vodret S., Piazza S., Rustighi A., Mantovani F., Belletti B., Baldassarre G., Blandino G., Tripodo C., Bicciato S., Mitro N, & Del Sal G. (2023). Mutant p53 sustains serine-glycine synthesis and essential amino acids intake promoting breast cancer growth. Nature Communications, 14, 6777.
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4

Ectopic Expression of TP53 Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following compounds and working concentrations were used: Fibronectin (10 μg/ml, Sigma Aldrich F0895), JPH203 (Selleck Chemicals S8667), NCT503 (Sigma Aldrich SML1659), Dasatinib (Selleck Chemicals S1021), PF-573228 (Selleck Chemicals S2013), Cytochalasin D (Sigma Aldrich C2618), Latrunculin-A (Cayman 10010630), SAHA Cayman 149647-78-9), 17-AAG Geldanamycin (Lc Laboratories A-6880), GCN2-IN-1 (Synonyms: A-92; MedChem Express Cat. No.: HY-100877), nucleosides dNTPs (Euroclone EMR276425), N-Acetyl-L-Cysteine (NAC) (Sigma Aldrich A9165), S-(5′-Adenosyl)-L-Methionine Iodide (SAM) (Sigma Aldrich A4377) and DMSO (Sigma Aldrich D4540). Treatments lasted as described in figure legends.
pCW57-GFP-P2A-MCS (empty backbone) was bought from Addgene (plasmid #89181). pCW57-GFP-HA-p53 wt, pCW57-GFP-HA-mutp53 R175H, pCW57-GFP-HA-mutp53 R249S, pCW57-GFP-HA-mutp53 R273K or pCW57-GFP-HA-mutp53 R280K were generated by cloning a PCR amplified DNA fragment of the human HA-tagged TP53 sequence (WT or mutated) into the pCW57-GFP-P2A-MCS with MluI and BamHI restriction sites.
Tombari C., Zannini A., Bertolio R., Pedretti S., Audano M., Triboli L., Cancila V., Vacca D., Caputo M., Donzelli S., Segatto I., Vodret S., Piazza S., Rustighi A., Mantovani F., Belletti B., Baldassarre G., Blandino G., Tripodo C., Bicciato S., Mitro N, & Del Sal G. (2023). Mutant p53 sustains serine-glycine synthesis and essential amino acids intake promoting breast cancer growth. Nature Communications, 14, 6777.
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