The cut piece of the WE was stored at −20 °C until DNA extraction. In total, 3 mL of treated WW were centrifuged for 10 min with 14,000g at 4 °C. The supernatant was removed and the pellet stored at −20 °C until DNA extraction. DNA was extracted using the NucleoSpin(R) Tissue Kit (Macherey Nagel, Düren, Germany). The WW pellet was redissolved in the first extraction buffer containing T1 + Proteinase K. The same buffer was also added to the WE piece. All samples were incubated at 56 °C for 2 h and then further processed according to the manufacturer’s recommendation.
Nucleospin r tissue kit
Manufactured by Macherey-Nagel
Sourced in Germany
The NucleoSpin(R) Tissue Kit is a laboratory product designed for the isolation and purification of DNA from various tissue samples. It utilizes a silica-based membrane technology to efficiently capture and purify DNA, making it suitable for downstream applications.
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Lab products found in correlation
2 protocols using nucleospin r tissue kit
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Wastewater DNA Extraction Protocol
2
DNA Extraction from Bacterial Isolates
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DNA extraction was performed on all isolates after inoculation in TSB media and incubation overnight at 37 • C, using the NucleoSpin R Tissue Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer's instructions.
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