Wheat germ agglutinin alexa fluor 555

Manufactured by Thermo Fisher Scientific

Sourced in United States

Wheat germ agglutinin Alexa Fluor 555 is a fluorescently labeled lectin that binds specifically to N-acetylglucosamine and sialic acid residues. It can be used to detect and visualize these carbohydrate moieties in various biological samples.

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Lab products found in correlation

Cho k1 cells, by American Type Culture Collection (1 mentions) Draq5 fluorescent probe solution, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 nhs ester, by Thermo Fisher Scientific (1 mentions) Glutamax supplement, by Thermo Fisher Scientific (1 mentions) Pierce ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions) Cytochalasin d, by Thermo Fisher Scientific (1 mentions) F 12k medium, by American Type Culture Collection (1 mentions) Fv1200 confocal microscope, by Olympus (1 mentions) Rat tail collagen, by Merck Group (1 mentions) Hoechst 33432, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Alexa Fluor 555 (1070 citations) Alexa 555 (299 citations) Alexa Fluors (21 citations) Alexa Fluor 555-conjugated wheat germ agglutinin (WGA; (8 citations) Alexa Fluor 555-conjugated wheat germ agglutinin (WGA) (4 citations) Wheat Germ Agglutinin Alexa Fluor 555 conjugate (3 citations)

2 protocols using wheat germ agglutinin alexa fluor 555

Inflammasome Activation Assay in THP-1 Cells

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The following reagents were purchased: phorbol 12-myristate 13-acetate (PMA) and CP-456773 sodium salt (CRID3) from Sigma-Aldrich (USA); Amicon Ultra 0.5 mL centrifugal filter MWCO 100 kDa, MF-Millipore membrane filters of 0.45 μm and 0.22 μm pore sizes from Millipore Sigma (USA); GM-CSF recombinant human protein, fetal bovine serum (FBS), penicillin-streptomycin, sodium pyruvate (100 mM), GlutaMAX supplement, RPMI 1640 medium, PBS buffer, Pierce LDH cytotoxicity assay kit, cytochalasin D (CytD), Alexa Fluor 488 NHS ester (succinimidyl ester), wheat germ agglutinin Alexa Fluor 555, DRAQ5 fluorescent probe solution (5 mM) and 4% formaldehyde from ThermoFisher Scientific (USA); acridine orange (AO) from Dojindo Laboratories (Kumamoto, Japan); VX765 and lipopolysaccharide (LPS) Escherichia coli O111:B4 from InvivoGen (USA); human IL1 beta assay kit from Cisbio Bioassays (France); CD14 microbeads (human) from Miltenyi Biotech (Germany); silica crystals (MIN-U-SIL-15) from US Silica (USA); μ-Slide 8 well ibiTreat from Martinsried (Germany); THP-1 cells from Japanese Collection of Research Bioresource Cell Bank (Japan); CHO-K1 cells and F-12K medium from ATCC (USA).
Human PBMCs were donated from University Hospital Bonn (Germany), and the operation and usage were approved by the local ethics committee in accordance with the Declaration of Helsinki.

He J., Li T., Próchnicki T., Horvath G., Latz E, & Takeoka S. (2019). Membrane fusogenic lysine type lipid assemblies possess enhanced NLRP3 inflammasome activation potency. Biochemistry and Biophysics Reports, 18, 100623.

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Cellular Uptake of Fluorescent Nanoparticles

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LabTek #1.5 borosilicate 8-well chamber slides were coated with 300 μL of rat tail collagen (Millipore Sigma) at a concentration of 50 μg/mL in 0.02N acetic acid for 5 minutes at room temperature. Chamber slides were washed once with PBS and air-dried for 5 minutes. 8,000 GBM22 cells were seeded per well in 300 μL media and allowed to adhere overnight before treatment with 5 μg/mL fluorescent NPs for 24 hours. Cells were carefully washed with HBSS on ice and incubated with 4 μg/mL wheat germ agglutinin-Alexa fluor 555 (Thermo Fisher) in ice-cold HBSS for 2 minutes. Cells were washed once with ice-cold PBS without incubation to remove excess wheat germ agglutinin and subsequently washed with PBS twice for 5 minutes before fixation with 4% paraformaldehyde (Pierce) for 15 minutes at room temperature. The cells were washed three more times with PBS and incubated with 4 μM Hoechst 33432 (Thermo Fisher) for 5 minutes at room temperature. Cells were imaged using an Olympus FV1200 confocal microscope with a 100x oil immersion objective. Images were pseudocoloured using FIJI.

Pickering A.J., Lamson N.G., Marand M.H., Hwang W., Straehla J.P, & Hammond P.T. (2023). Layer-by-Layer Polymer Functionalization Improves Nanoparticle Penetration and Glioblastoma Targeting in the Brain. ACS nano, 17(23).

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