Phosphorylation of NF-κB p65 at serine 536 in pCECs after activation was investigated by flow cytometry as previously described 42 (link). Briefly, pCECs were harvested 5 minutes after stimulation with hTNF-α, fixed, permeabilized, and incubated successively with anti-phospho NF-κB p65 antibody (Cell Signaling, Danvers, MA), followed by staining with phycoerythrin-conjugated anti-rabbit IgG antibody (Cell Signaling). Rabbit IgG antibody (isotype) (Cell Signaling) was used as a negative control.
Rabbit igg antibody isotype
Manufactured by Cell Signaling Technology
Rabbit IgG antibody (isotype) is a laboratory reagent used in various scientific applications. It is a purified immunoglobulin G (IgG) antibody derived from rabbit serum. The core function of this product is to serve as a control or reference antibody in experimental procedures where rabbit-derived antibodies are utilized.
Automatically generated - may contain errors
2 protocols using rabbit igg antibody isotype
1
Phosphorylation of NF-κB p65 in pCECs
2
NF-κB p65 Phosphorylation in AECs
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