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Pe cyanine7

Manufactured by BD
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Sourced in United States

PE-Cyanine7 is a fluorescent dye that can be used for flow cytometry and other fluorescence-based applications. It excites at 488 nm and emits at 767 nm, making it suitable for detection in the far-red spectrum.

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Lab products found in correlation

Alexa fluor 488, by BD (2 mentions) Rneasy micro kit, by Qiagen (1 mentions) Cd25 apc, by BD (1 mentions) Fastquant rt kit, by Tiangen Biotech (1 mentions) Leukocyte activation cocktail, by BD (1 mentions) Superreal premix plus, by Tiangen Biotech (1 mentions) Cd45ra pe, by BD (1 mentions) Sybr green, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Fluorescein isothiocyanate (fitc), by BD (1 mentions) Rorγt, by BD (1 mentions) Gm csf, by BD (1 mentions) Alexa fluor 647, by BD (1 mentions) Permeabilization solution, by BD (1 mentions) Tnf α, by BD (1 mentions) Mcp 1, by BD (1 mentions) Dapta, by Bio-Techne (1 mentions) Nf κb p65, by BD (1 mentions) Notch1, by BD (1 mentions) Lsrfortessa cell analyzer, by BD (1 mentions)

Close spelling variants of this product

CD8-PE-Cyanine7 (2 citations)

3 protocols using pe cyanine7

1

Murine Immune Cell Immunophenotyping

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Anti-mouse rat antibodies (conjugated with FITC, PE, PE-Cyanine7, PE-CF594, PerCP, APC, Alexa Fluor® 488, and BV510 as indicated) used in this study were: CD4-PerCP (L3T4, BD Pharmingen™, United States), CD25-APC (IL-2 Receptor α chain, BD Pharmingen™, United States), FoxP3-PE-Cyanine7 (JM2, eBioscience, United States), CD45RA-PE (BD Pharmingen™, United States), IL-17-Alexa Fluor® 488 (BD Pharmingen™, United States), CD3e-PE-CF594 (CD3ε chain, BD Horizon™, United States), CD8a-BV510 (Ly-B, BD Horizon™, United States). In all experiments, a control antibody of the respective IgG isotype was included. Leukocyte Activation Cocktail (BD Pharmingen™, United States), RNeasy Micro Kit (QIAGEN, Germany), FastQuant RT Kit (TIANGEN, China), SuperReal PreMix Plus (TIANGEN, China), and the Mouse Th1/Th2/Th17 Cytokine kit (BD Biosciences, United States) were used according to the manufacturer’s instructions.
Ma Y.H., Zhang J., Chen X., Xie Y.F., Pang Y.H, & Liu X.J. (2016). Increased CD4+CD45RA-FoxP3low cells alter the balance between Treg and Th17 cells in colitis mice. World Journal of Gastroenterology, 22(42), 9356-9367.
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2

Multiparametric Immunophenotyping and qRT-PCR

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DAPTA was purchased from Tocris Bioscience (Bristol, UK). Fluorescein isothiocyanate, PE/Cyanine7, Allophycocyanin, Alexa Fluor® 488, PE/Dazzle™ 594, Alexa Fluor® 647, and phycoerythrin-labeled CD40, NF-κB p65, IκBα, Notch1, Notch3, GM-CSF, iNOS, RORγT, MCP-1, TNF-α, lysis, and permeabilization solution, and fixation buffers were purchased from BD Biosciences and BioLegend (San Diego, CA, USA). SYBR Green and cDNA kits were purchased from Applied Biosystems (Foster City, CA, USA). Primers were purchased from GenScript (Piscataway, NJ, USA). TRizol was purchased from Invitrogen (Carlsbad, CA, USA).
Alghibiwi H., Ansari M.A., Nadeem A., Algonaiah M.A., Attia S.M., Bakheet S.A., Albekairi T.H., Almudimeegh S., Alhamed A.S., Shahid M., Alwetaid M.Y., Alassmrry Y.A, & Ahmad S.F. (2023). DAPTA, a C-C Chemokine Receptor 5 (CCR5), Leads to the Downregulation of Notch/NF-κB Signaling and Proinflammatory Mediators in CD40+ Cells in Experimental Autoimmune Encephalomyelitis Model in SJL/J Mice. Biomedicines, 11(6), 1511.
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3

Immunophenotypic Characterization of Cultured eMSCs

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The cultured eMSCs and MSCs were subjected to flow cytometric analysis. In brief, aliquots of 1 × 106 cells per mL were immunolabeled at RT for 30 min with the following antibodies: anti-human CD34, CD45, and CD73 antibodies conjugated to PerCP-Cy5.5 and anti-human CD105 antibodies conjugated to PE-Cyanine 7 (BD Biosciences, San Jose, CA, USA). Cells were then acquired using an LSR Fortessa cell analyzer (BD Biosciences). The data were analyzed using the FlowJo 7.6.5 software (TreeStar Inc., Ashland, OR, USA).
Kim J.W., Park N., Kang J., Kim Y., Jung H., Rim Y.A, & Ju J.H. (2021). Increased Potential of Bone Formation with the Intravenous Injection of a Parathyroid Hormone-Related Protein Minicircle DNA Vector. International Journal of Molecular Sciences, 22(16), 9069.
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