Reagents: TaqMan MicroRNA assay kit (provided by Applied Biosystems; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and PrimeScript™ RT reagent kit, TRIzol, fetal bovine serum and agar medium (Promega Corporation, Madison, WI, USA) and PCR kit (Jianlun Science and Technology Co., Guangdong, China) were used in the current study. Instruments used were: ultra-low-temperature refrigerator (Sanyo, Osaka, Japan), 7500 HT real-time polymerase chain reaction (PCR) detector, and pure water and water purifier (Angel Water, Inc., Barrington, IL, USA).
Ultra low temperature refrigerator
Manufactured by Sanyo
Sourced in Japan
The Ultra-low-temperature refrigerator is a laboratory equipment designed to maintain extremely low temperatures, typically ranging from -40°C to -86°C. Its core function is to provide a controlled environment for the storage and preservation of temperature-sensitive samples, materials, or experiments.
Automatically generated - may contain errors
Lab products found in correlation
Primescript rt reagent kit, by Promega (1 mentions)
Taqman microrna assay kit, by Thermo Fisher Scientific (1 mentions)
Trizol, by Promega (1 mentions)
Ultraviolet spectrophotometer, by Bio-Rad (1 mentions)
Ch2 optical microscope, by Olympus (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Autoclave, by TOMY (1 mentions)
Triglyceride tg test kit, by Nanjing Jiancheng (1 mentions)
Total cholesterol test kit, by Nanjing Jiancheng (1 mentions)
Superoxide dismutase, by Nanjing Jiancheng (1 mentions)
Malondialdehyde mda test kit, by Nanjing Jiancheng (1 mentions)
Sod test kit, by Nanjing Jiancheng (1 mentions)
Ultrapure water meter, by Merck Group (1 mentions)
Diaminobenzidine hydrochloride dab, by Merck Group (1 mentions)
Cx31 microscope, by Olympus (1 mentions)
Electronic balance, by Sartorius (1 mentions)
4 protocols using ultra low temperature refrigerator
1
Quantitative Analysis of miRNA Expression
2
Immune Response in Genetically Modified Mice
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Male 5-year-old C67/B7 mice and male 5-year-old CARD9-/- C67/B7 mice were adopted. The instruments used in the experiment were as follows: fully automatic mild tissue processor (140-073-285) (Miltenyi); autoclave (TOMY); biosafety cabinet (Baker); cell incubator (Thermo Forma); refrigerated centrifuge (Heraeus); ultra-low temperature refrigerator (SANYO); oven (Heraeus); pure water meter; FACS Calibur flow cytometer (BD); CO2 incubator (ThermoForma); Step One Plus fluorescence ration PCR instrument (ABI); CH2 optical microscope (Olympus); microplate reader (BIO-RAD); nucleic acid level electrophoresis apparatus (Bio-Rad); ultraviolet spectrophotometer (Bio-Rad); Gel Doc EQ MultiImager (Bio-Rad); Step One Plus fluorescence ration PCR instrument (ABI).
3
Carrot Pulp Fermentation and Bioactive Analysis
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Ordinarily fermented and rebiotics-fermented carrot pulps are used [35 ]. Ampicillin sodium (0.5 g/mL) is from Shanghai Shenggong Bioengineering Co. Ltd. Total cholesterol (TC) test kit, triglyceride (TG) test kit, malondialdehyde (MDA) test kit, superoxide dismutase (SOD) test kit, and reduced glutathione (GSH) test kit are from Nanjing Jiancheng Bioengineering Institute.
Electronic analysis balance is obtained from Yuyao Jinnuo Tianping Instrument Co., Ltd.; Pipette from Shanghai Thermo Fisher Scientific Instrument Co. Ltd.; Ultra-low temperature refrigerator from SANYO (Japan); and 1500-823 microplate reader from Thermo Fisher Scientific.
Electronic analysis balance is obtained from Yuyao Jinnuo Tianping Instrument Co., Ltd.; Pipette from Shanghai Thermo Fisher Scientific Instrument Co. Ltd.; Ultra-low temperature refrigerator from SANYO (Japan); and 1500-823 microplate reader from Thermo Fisher Scientific.
4
Detailed Cultivation and Identification of H. pylori Strains
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Sydney strain 1, H. pylori SS1 (VacA+, CagA-), and Hp49503 (VacA+, CagA+) strains were purchased from the Department of Pathogenic Biology, School of Medicine, Shanghai Jiao Tong University. Brucella Broth was provided by the Municipal Center for Disease Control and Prevention of Shanghai. Agar powder was purchased from Oxoid (UK). Simple culture tank with ventilation was prepared by the Shanghai Academy of Life Sciences, Chinese Academy of Sciences. Others included Gram staining kit (Shanghai Kang'en Biotech Co. Ltd), H. pylori detection kit (urease method) (Zhuhai Lituo Biotech Co. Ltd), diaminobenzidine hydrochloride (DAB) (Sigma, USA), mixed gas (85% N2, 10% CO2, 5% O2) (Shanghai Youjiali Liquid Nitrogen Co. Ltd.), high-pressure steam sterilizer (SANYO, Japan), ultraclean workbench (Suzhou Yongjiu Pressure Regulator Factory), CX31 microscope (Olympus, Japan), electronic balance (Sartorius, Germany), RM2145 slicer (Leica, Germany), ultralow temperature refrigerator (SANYO, Japan), CD177 antibody (ab8092; Abcam), UV inhibitor (Roche Diagnostics), UV UNIV MULT HRP (Roche Diagnostics), carbolic acid red dye solution (Beijing Leigen Biotech Co. Ltd), ultrapure water meter (Millipore, USA), electric thermostatic water bath (Shanghai Yiheng Co. Ltd), and benchtop horizontal centrifuge (Eppendorf, Germany).
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