Pacgp67 a vector

Manufactured by BD

Sourced in United States

The PAcGP67-A vector is a plasmid used for the expression of recombinant proteins in insect cell lines. It contains an Autographa californica nucleopolyhedrovirus (AcNPV) immediate-early gene promoter (P67) to drive the expression of the gene of interest. The vector also includes antibiotic resistance genes for selection in bacteria.

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Lab products found in correlation

Superdex 200 column, by GE Healthcare (6 mentions) Nickel agarose, by Qiagen (5 mentions) Biowhittaker insect xpress media, by Lonza (5 mentions) Sf900ii media, by Thermo Fisher Scientific (5 mentions) Insect xpress media, by Lonza (2 mentions) Hispur ni nta resin, by Thermo Fisher Scientific (1 mentions) Histrap hp column, by GE Healthcare (1 mentions) Tni insect cells, by Expression Systems (1 mentions) Monos column, by GE Healthcare (1 mentions) Superdex 200 10 300 gl column, by GE Healthcare (1 mentions) Q sepharose anion exchange chromatography, by GE Healthcare (1 mentions) Ni nitrilotriacetic acid agarose resin, by Qiagen (1 mentions) Nanodrop 2000 spectrometer, by Thermo Fisher Scientific (1 mentions) Nickel nta agarose, by Qiagen (1 mentions)

Close spelling variants of this product

PAcGP67

10 protocols using pacgp67 a vector

Expression and Purification of IL-2 Variants and Receptors

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Example 3

Human IL-2 variants (amino acids 1-133), the IL-2Rβ ectodomain (amino acids 1-214), and γ_c(amino acids 34-232) were cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and C-terminal hexahistidine tag and produced using the baculovirus expression system. Baculovirus stocks were prepared by transfection and amplification in Spodoptera frugiperda (Sf9) cells grown in SF9000II media (Invitrogen), and protein expression was carried out in suspension Trichoplasia ni (High Five™) cells grown in Bio Whittaker® Insect XPRESS™ media (Lonza). Proteins were expressed and captured from High Five™ supernatants after 48-60 hr by nickel agarose (QIAGEN), concentrated and purified by size exclusion chromatography on a Superdex™ 200 column (GE Healthcare), equilibrated in 10 mM HEPES (pH 7.2) and 150 mM NaCl. IL-2 variants used in SPR and cell based assays were expressed fully glycosylated. For biotinylated receptor expression, IL-2Rβ and γ_cwere cloned into the pAcGP67-A vector with a C-terminal biotin acceptor peptide (BAP)-LNDIFEAQKIEWHE and hexahistidine tag. Receptor proteins were coexpressed with BirA ligase with excess biotin (100 uM).

US10183980B2. Superagonists and antagonists of interleukin-2 (2019-01-22). The Board of Trustees of the Leland Stanford Junior University, Office of the General Counsel [US]. Inventors: Kenan Christopher Garcia [US], Aron Levin [US], Aaron Ring [US].

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Recombinant human IL-2 variants production

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Example 3

Human IL-2 variants (amino acids 1-133), the IL-2Rβ ectodomain (amino acids 1-214), and γ_c(amino acids 34-232) were cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and C-terminal hexahistidine tag and produced using the baculovirus expression system. Baculovirus stocks were prepared by transfection and amplification in Spodoptera frugiperda (Sf9) cells grown in SF900II media (Invitrogen), and protein expression was carried out in suspension Trichoplusia ni (High Five™) cells grown in BioWhittaker® Insect XPRESS™ media (Lonza). Proteins were expressed and captured from High Five™ supernatants after 48-60 hr by nickel agarose (QIAGEN), concentrated and purified by size exclusion chromatography on a Superdex™ 200 column (GE Healthcare), equilibrated in 10 mM HEPES (pH 7.2) and 150 mM NaCl. IL-2 variants used in SPR and cell based assays were expressed fully glycoslylated. For biotinylated receptor expression, IL-2Rβ and 7e were cloned into the pAcGP67-A vector with a C-terminal biotin acceptor peptide (BAP)-LNDIFEAQKIEWHE and hexahistidine tag. Receptor proteins were coexpressed with BirA ligase with excess biotin (100 uM).

US11384131B2. Superagonists, partial agonists and antagonists of interleukin-2 (2022-07-12). The Board of Trustees of the Leland Stanford Junior University [US], United States Department of Health and Human Services, Office of Technology Transfer, National Institutes of Heath [US]. Inventors: Christopher K. Garcia [US], Suman Mitra [US], Warren J. Leonard [US], Aaron M. Ring [US].

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Recombinant HyIL-6 Protein Production

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HyIL-6 (Fischer et al., 1997 (link)) cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and a C-terminal hexahistine tag was produced using the baculoviral expression system, as previously described (LaPorte et al., 2008 (link)). The baculoviral stocks were prepared in Spodoptera frugiperda (Sf9) cells grown in SF900III media (Invitrogen, #12658027) and used to infect Trichoplusiani ni (High Five) cells grown in InsectXpress media (Lonza, #BELN12-730Q) for protein expression. After 48h infection, secreted protein was captured from High Five supernatants using HisPur Ni-NTA resin (Thermo Scientific, #88223) affinity chromatography, concentrated, and purified by size exclusion chromatography on a Enrich SEC 650 1 × 300 column (Biorad), equilibrated in 10 mM HEPES (pH 7.2) containing 150 mM NaCl. Recombinant HyIL-6 was purified to greater than 98% homogeneity.

Martinez-Fabregas J., Wang L., Pohler E., Cozzani A., Wilmes S., Kazemian M., Mitra S, & Moraga I. (2020). CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci. Cell Reports, 33(12), 108545.

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Production and Purification of IL-2 Variants and Receptors

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Example 3

Human IL-2 variants (amino acids 1-133), the IL-2Rβ ectodomain (amino acids 1-214), and γ_c(amino acids 34-232) were cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and C-terminal hexahistidine tag and produced using the baculovirus expression system. Baculovirus stocks were prepared by transfection and amplification in Spodoptera frugiperda (Sf9) cells grown in SF900II media (Invitrogen), and protein expression was carried out in suspension Trichoplusia ni (High Five™) cells grown in BioWhittaker® Insect XPRESS™ media (Lonza). Proteins were expressed and captured from High Five™ supernatants after 48-60 hr by nickel agarose (QIAGEN), concentrated and purified by size exclusion chromatography on a Superdex™ 200 column (GE Healthcare), equilibrated in 10 mM HEPES (pH 7.2) and 150 mM NaCl. IL-2 variants used in SPR and cell based assays were expressed fully glycoslylated. For biotinylated receptor expression, IL-2Rβ and γ_cwere cloned into the pAcGP67-A vector with a C-terminal biotin acceptor peptide (BAP)-LNDIFEAQKIEWHE and hexahistidine tag. Receptor proteins were coexpressed with BirA ligase with excess biotin (100 uM).

US10150802B2. Superagonists, partial agonists and antagonists of interleukin-2 (2018-12-11). THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY [US], THE UNITED STATE OF AMERICA DEPARTMENT OF HEALTH AND HUMAN SERVICES, OFFICE OF TECHNOLOGY TRANSFER, NATIONAL INSTITUTES OF HEALTH [US]. Inventors: Christopher K. Garcia [US], Suman Mitra [US], Warren J. Leonard [US], Aaron M. Ring [US].

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Expression and Purification of mTLR9-cECD

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The truncated C-terminal ectodomain fragment of mouse TLR9 (mTLR9-cECD) was expressed in a baculovirus-insect cell system and purified as described previously (Li et al, 2012 (link)). Briefly, a gene encoding the proteolytically processed fragment (residues 474–824) mTLR9-cECD with an N-terminal eight-histidine purification tag, followed by the linker sequence Ser-Ser-Gly and a tobacco etch virus protease cleavage site, was cloned into the pAcGP67-A vector (BD Biosciences) in frame with the baculovirus gp67 signal sequence. mTLR9-cECD was expressed in Tni insect cells (Expression Systems). Cells were infected with 1% (v/v) of third-passage baculovirus stock. After culture in suspension for 72 h at 27°C mTLR9-cECD was extracted from intracellular compartments with 50 mM Tris pH 7.5, 500 mM NaCl, 10% glycerol, 5 mM β-mercaptoethanol, 1% Fos-Choline 12. mTLR9-cECD was purified by nickel affinity chromatography with a HisTrap HP column (GE Healthcare), followed by cation exchange chromatography with a MonoS column (GE Healthcare) and size-exclusion chromatography with a Superdex 200 10/300 GL column (GE Healthcare). The size-exclusion buffer was 20 mM MES pH 5.6, 100 mM NaCl, 2 mM β-mercaptoethanol.

Rigby R.E., Webb L.M., Mackenzie K.J., Li Y., Leitch A., Reijns M.A., Lundie R.J., Revuelta A., Davidson D.J., Diebold S., Modis Y., MacDonald A.S, & Jackson A.P. (2014). RNA:DNA hybrids are a novel molecular pattern sensed by TLR9. The EMBO Journal, 33(6), 542-558.

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Expression and Purification of IL-2 Variants and Receptors

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Example 3

Human IL-2 variants (amino acids 1-133), the IL-2Rβ ectodomain (amino acids 1-214), and γ_c(amino acids 34-232) were cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and C-terminal hexahistidine tag and produced using the baculovirus expression system. Baculovirus stocks were prepared by transfection and amplification in Spodoptera frupperda (Sf9) cells grown in SF900II media (Invitrogen), and protein expression was carried out in suspension Trichoplusia ni (High Five™) cells grown in BioWhittaker® Insect XPRESS™ media (Lonza). Proteins were expressed and captured from High Five™ supernatants after 48-60 hr by nickel agarose (QIAGEN), concentrated and purified by size exclusion chromatography on a Superdex™ 200 column (GE Healthcare), equilibrated in 10 mM HEPES (pH 7.2) and 150 mM NaCl. IL-2 variants used in SPR and cell based assays were expressed fully glycoslylated. For biotinylated receptor expression, IL-2Rβ and γ_cwere cloned into the pAcGP67-A vector with a C-terminal biotin acceptor peptide (BAP)-LNDIFEAQKIEWHE and hexahistidine tag. Receptor proteins were coexpressed with BirA ligase with excess biotin (100 uM).

US10654905B2. Method of treating graft versus host disease with an interleukin-2 mutein (2020-05-19). The Board of Trustees of the Leland Stanford Junior University [US], United States Department of Health and Human Services, Office of Technology Transfer, National Institutes of Health [US]. Inventors: Christopher K. Garcia [US], Suman Mitra [US], Warren J. Leonard [US], Aaron M. Ring [US].

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Recombinant Cytokine and Receptor Production

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Example 3

Human IL-2 variants (amino acids 1-133), the IL-2R13 ectodomain (amino acids 1-214), and γ_c(amino acids 34-232) were cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and C-terminal hexahistidine tag and produced using the baculovirus expression system. Baculovirus stocks were prepared by transfection and amplification in Spodoptera frugiperda (Sf9) cells grown in SF900II media (Invitrogen), and protein expression was carried out in suspension Trichoplusia ni (High Five™) cells grown in BioWhittaker® Insect XPRESS™ media (Lonza). Proteins were expressed and captured from High Five™ supernatants after 48-60 hr by nickel agarose (QIAGEN), concentrated and purified by size exclusion chromatography on a Superdex™ 200 column (GE Healthcare), equilibrated in 10 mM HEPES (pH 7.2) and 150 mM NaCl. IL-2 variants used in SPR and cell based assays were expressed fully glycoslylated. For biotinylated receptor expression, IL-2Rβ and γ_cwere cloned into the pAcGP67-A vector with a C-terminal biotin acceptor peptide (BAP)-LNDIFEAQKIEWHE and hexahistidine tag. Receptor proteins were coexpressed with BirA ligase with excess biotin (100 uM).

US11117943B2. Superagonists and antagonists of interleukin-2 (2021-09-14). The Board of Trustees of the Leland Stanford Junior University [US]. Inventors: Kenan Christopher Garcia [US], Aron Levin [US], Aaron Ring [US].

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Recombinant Influenza Protein Production

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The wt NA and mutant NA (I223R/H275Y) genes derived from A/H1N1 2009 pandemic influenza virus were amplified by PCR. The PCR products were cloned into the pAcGP67A vector (BD Biosciences) and transfected into the Sf9 insect cell line to produce recombinant proteins. The culture medium was collected, clarified by centrifugation at 110 × g, and subjected to further viral propagation. The cultured Sf9 cells were inoculated with recombinant baculovirus at a multiplicity of infection of 10 and incubated at 27 °C for 96 h. The cells were collected after centrifugation, and the cell pellet was resuspended in cell lysis buffer (50 mM Tris-HCl, pH 8.5, 5 mM 2-mercaptoethanol, 100 mM KCl, 1 mM phenylmethylsulfonyl fluoride), sonicated, and centrifuged at 16,000 × g for 15 min at 4 °C. After the cell lysate was sonicated to reduce its viscosity, the cell debris was removed by centrifugation for 1 h at 16,000 × g. The soluble protein from the cell supernatant was applied to Ni-nitrilotriacetic acid agarose resin (Qiagen), washed, and eluted with buffer (50 mM Tris-HCl, 0.5 M NaCl, 0.5 M imidazole, pH 8.0). The purified proteins were dialyzed against phosphate-buffered saline (PBS) and further purified by Q-Sepharose anion-exchange chromatography (GE Healthcare).

Guk K., Kim H., Lee M., Choi Y.A., Hwang S.G., Han G., Kim H.N., Kim H., Park H., Yong D., Kang T., Lim E.K, & Jung J. (2020). Development of A4 antibody for detection of neuraminidase I223R/H275Y-associated antiviral multidrug-resistant influenza virus. Nature Communications, 11, 3418.

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Recombinant Expression of EPDR1 Homologs

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DNAs for human EPDR1 (UniProt ID Q9UM22; residues 38–224), mouse EPDR1 (UniProt ID Q99M71; residues 38–224) and frog EPDR1 (X. tropicalis; NCBI Accession No. XP_002939463; residues 38–220), which all exclude the native N-terminal signal-peptide sequences, were gene-synthesized (Bioneer, Daejeon, Republic of Korea) with the addition of N-terminal His₈ tags and BamHI/NotI restriction-enzyme sites. The genes were also codon-optimized for expression in the Spodoptera frugiperda (Sf9) insect-cell line. The synthesized DNAs were subcloned into pAcGP67A vector (BD Biosciences, Franklin Lakes, New Jersey, USA) for secreted expression.

Park J.K., Kim K.Y., Sim Y.W., Kim Y.I., Kim J.K., Lee C., Han J., Kim C.U., Lee J.E, & Park S. (2019). Structures of three ependymin-related proteins suggest their function as a hydrophobic molecule binder. IUCrJ, 6(Pt 4), 729-739.

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Recombinant IL13 and IL13 Receptor Expression

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Human IL13 and the IL13 variants were cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and a C-terminal hexahistidine tag and produced using the baculovirus expression system, as described in LaPorte et al. (34 (link)). Baculovirus stocks were prepared by transfection and amplification in Spodoptera frugiperda (Sf9) cells grown in SF900II media (Invitrogen), and protein expression was carried out in suspension Trichoplusiani (High Five) cells grown in InsectXpress media (Lonza). Following expression, proteins were captured from High Five supernatants after 48 h by nickel-nitrilotriacetic acid (nickel-NTA) agarose (Qiagen) affinity chromatography, concentrated, and purified by size exclusion chromatography on a Superdex 200 column (GE Healthcare), equilibrated in 10 mM HEPES (pH 7.2) containing 150 mM NaCl (CAS 7647-14-5). Recombinant cytokines were purified to greater than 98% homogeneity. For biotinylated receptor expression, IL13Rα1/IL13Rα2 ectodomains were cloned into the pAcGP67-A vector with C-terminal biotin acceptor peptide LNDIFEAQKIEWHW followed by a hexahistidine tag. Receptors were coexpressed with BirA ligase in the presence of excess biotin (10 µM). Protein concentrations were quantified by ultraviolet (UV) spectroscopy at 280 nm using a Nanodrop2000 spectrometer (Thermo Fisher Scientific, RRID: SCR_018042).

Stern L.A., Gholamin S., Moraga I., Yang X., Saravanakumar S., Cohen J.R., Starr R., Aguilar B., Salvary V., Hibbard J.C., Kalbasi A., Shepphird J.K., O’Hearn J., Garcia K.C, & Brown C.E. (2022). Engineered IL13 variants direct specificity of IL13Rα2-targeted CAR T cell therapy. Proceedings of the National Academy of Sciences of the United States of America, 119(33), e2112006119.

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