CMSCs were used for the endothelial cell induction protocol. Cells were seeded at a density of 3,000 cells per cm2 on 24-well plates in EGM-2 culture medium with supplements (Lonza, Walkersville, MD, USA) and cultured for 10 days. The expression of CD31, an endothelial cell marker, was then evaluated by immunofluorescence (BD Biosciences). On the following day, sections were incubated with Alexa fluor 594-conjugated anti-goat IgG (Molecular Probes, Carlsbad, CA, USA). To induce the formation of capillary-like structures, 24-well plates were covered with 250 μL of Matrigel (BD Biosciences) diluted 1:1 in EGM-2. CMSCs were seeded at a density of 30,000 cells per cm2 and cultured for 24 hours. The formation of capillary-like structures was observed over time by using an inverted microscope (Olympus). Additionally, the percentage of CD31 cells before and after EGM-2 induction was evaluated by flow cytometry with APC-conjugated anti-CD31 (BD Biosciences) diluted 1:50 and by analysis with the LSRFortessa flow cytometer.
Apc conjugated anti cd31
Manufactured by BD
Sourced in United States
APC-conjugated anti-CD31 is a fluorescent-labeled antibody that binds to the CD31 (PECAM-1) antigen expressed on the surface of endothelial cells. It can be used in flow cytometry applications to identify and analyze endothelial cells.
Automatically generated - may contain errors
Lab products found in correlation
Egm 2 culture medium, by Lonza (1 mentions)
Alexa fluor 594 conjugated anti goat igg, by Thermo Fisher Scientific (1 mentions)
Matrigel, by BD (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
Pe conjugated anti cd90, by BD (1 mentions)
Fitc conjugated anti cd29, by BD (1 mentions)
Fitc conjugated anti cd44, by BD (1 mentions)
Fitc conjugated anti cd45, by BD (1 mentions)
Accuri c6 cytometer, by BD (1 mentions)
2 protocols using apc conjugated anti cd31
1
Endothelial Cell Induction and Capillary-like Structure Formation
2
Flow Cytometry Characterization of ASCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To confirm the ASC-progenitor phenotype, flow cytometry was performed after 3 days following the protocol in the previous subsection. Briefly, after tissue digestion ASCs (8 × 10 4 ) were washed, resuspended in 1% BSA in PBS, and incubated with FITC-conjugated anti-CD29, APC-conjugated anti-CD31, FITC-conjugated anti-CD44, FITCconjugated anti-CD45, and PE-conjugated anti-CD90 (BD Bioscience, San Jose, CA,USA), respectively, for 1 h at 4°C. Corresponding isotype-matched antibodies were used as controls. FACS analysis was performed using an Accuri C6 cytometer (BD, Franklin Lakes, NJ, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!