Caspase 1

Manufactured by Beyotime

Sourced in China

Caspase-1 is a laboratory research tool used in the study of programmed cell death, or apoptosis. It is an enzyme that plays a critical role in the inflammatory response and the initiation of cell death pathways. Caspase-1 is responsible for the proteolytic processing and activation of inflammatory cytokines, such as interleukin-1β and interleukin-18. This product is intended for use in scientific research and not for diagnostic or therapeutic purposes.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using caspase 1

Western Blot Analysis of Inflammatory Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The proteins were extracted from the cells with radioimmunoprecipitation assay (RIPA) buffer (Beyotime Biotechnology), and the concentration was measured using a bicinchoninic acid (BCA) kit (Beyotime Biotechnology). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed to separate proteins and the cells were then transferred to the polyvinylidene fluoride membranes (PVDF; Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% bovine serum albumin (BSA) for 1 hour and incubated with TLR-4 (1:500, Beyotime Biotechnology), NLRP3 (1:500, Beyotime Biotechnology), p-p65 (1:500, Beyotime Biotechnology), Caspase-1 (1:500, Beyotime Biotechnology), or GAPDH (1:500, Beyotime Biotechnology) primary antibodies at 4 ℃ overnight. Subsequently, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:500, Beyotime Biotechnology) at room temperature for 1 hour, and then the bounded secondary antibody was visualized using chemiluminescence (Beyotime Biotechnology).

Kong L.Y., Zhu S.Y., Si M.Y., Xu X.H., Yu J.J., Zhong W.X., Sang C.P., Rao D.Y., Xie F.C., Liu Z.Y, & Tang Z.X. (2023). TAK-242 protects against oxygen-glucose deprivation and reoxygenation-induced injury in brain microvascular endothelial cells and alters the expression pattern of lncRNAs. Journal of Thoracic Disease, 15(5), 2571-2584.

+ Open protocol

+ Expand

Curcumin Inhibits Aflatoxin B1-Induced Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Curcumin (CAS: 458-37-7) was obtained from Nanjing NutriHerb BioTech Co., Ltd. (Nanjing, China). AFB1 (CAS no. 1162-65-8) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Antibodies were obtained from Beyotime Biotechnology (Shanghai, China) including GAPDH (catalog number: AG019), caspase-1 (catalog number: AF1681), NLRP3 (catalog number: AF2155), horseradish peroxidase (HRP)-labeled Goat Anti-Mouse IgG (catalog number: A0216) and HRP-labeled Goat Anti-Rabbit IgG (catalog number: A0208).

Jin S., Yang H., Wang Y., Pang Q., Jiao Y., Shan A, & Feng X. (2021). Dietary Curcumin Alleviated Aflatoxin B1-Induced Acute Liver Damage in Ducks by Regulating NLRP3–Caspase-1 Signaling Pathways. Foods, 10(12), 3086.

+ Open protocol

+ Expand

Elucidating Antiviral Immune Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

IFN-α/β, IL-1β, and IL-18 ELISA kits were purchased from Shanghai mlbio. Caspase-1, caspase-3, caspase-8 activity assay kits, Total Nitric Oxide (NO) assay kit, Enhanced ATP assay kit, Enhanced mitochondrial membrane potential assay kit, and PI3K inhibitor (LY294002) were purchased from Beyotime Institute of Biotechnology. Recombinant chicken IFN-α was purchased from Cloud clone crop (CCC, USA). Lipofectamine 3000 was purchased from Invitrogen. The following antibodies were used for immunoblot analysis: anti-ALV-J envelope protein JE9 (kindly provided by Dr. Aijian Qin, Yangzhou University, Yangzhou, China), anti-Flag (AF5051, Beyotime), FITC-labeled goat anti-rabbit IgG (H+L) (A0562, Beyotime), anti-STAT1 (70R-51641, Fitzgerald), anti-phosphorylated STAT1 (15H13L67, Invitrogen), anti-Akt (10176-2-AP, Proteintech), anti-phosphorylated Akt (D9E, Cell Signaling), anti-mTOR (bs-1992R, Bioss), anti-phosphorylated mTOR (D9C2, Cell Signaling), anti-IKKα/β (bs-10123R, Bioss), anti-phosphorylated IKK (bs-3229R, Bioss), anti-β-actin (AF5003, Beyotime), Goat anti-rabbit IgG/HRP (bs-0295G, Bioworld), Goat anti-mouse IgG/HRP (bs-0296G, Bioworld).

Zhang Q., Xie T., Mo G., Zhang Z., Lin L, & Zhang X. (2021). ACSL1 Inhibits ALV-J Replication by IFN-Ⅰ Signaling and PI3K/Akt Pathway. Frontiers in Immunology, 12, 774323.

+ Open protocol

+ Expand

Caspase Activity Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were lysed, and Caspase-1, Caspase-3, Caspase-8, and Caspase-9 kits (Beyotime, C1101, C1115, C1151, and C1157) were used to detect the activity of Caspase proteins in the cell supernatant and mouse serum. The absorbance was read at a 405 nm wavelength.

He X., Li C., Yin H., Tan X., Yi J., Tian S., Wang Y, & Liu J. (2022). Mesenchymal stem cells inhibited the apoptosis of alveolar epithelial cells caused by ARDS through CXCL12/CXCR4 axis. Bioengineered, 13(4), 9060-9070.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!