All blood sampling was performed at the General Clinical Research Center and analyzed at VUMC central laboratories. After blood draw was performed, samples were transported on ice and centrifuged at 3000 rpm for 15 min before being kept frozen at −80° Celsius. Plasma fasting glucose concentrations were analyzed using the glucose oxidase method (Glucose analyzer 2; Beckman Coulter, Brea, CA). Biochemistry measurements were analyzed at the VUMC Pathology Laboratory. High sensitivity C‐reactive protein (hs‐CRP) concentrations were measured by high‐sensitivity particle‐enhanced turbidimetric UniCel DxI Immunoassay system (Beckman Coulter).
Unicel dxi immunoassay system
Manufactured by Beckman Coulter
Sourced in United States
The UniCel DxI Immunoassay system is a fully automated instrument designed for high-throughput immunoassay testing. It is capable of performing a wide range of immunoassay tests, including hormone, therapeutic drug, and special chemistry analyses.
Automatically generated - may contain errors
Lab products found in correlation
Glucose analyzer 2, by Beckman Coulter (4 mentions)
Access accutni 3 troponin 1 assay, by Beckman Coulter (1 mentions)
Cytometric bead array, by BD (1 mentions)
Chemiluminescent immunoassay, by Beckman Coulter (1 mentions)
Triphenyl tetrazolium chloride (ttc), by Merck Group (1 mentions)
Ie33 ultrasound device, by Philips (1 mentions)
X3 1 transducer, by Philips (1 mentions)
Enzyme linked immunosorbent assay, by R&D Systems (1 mentions)
9 protocols using unicel dxi immunoassay system
1
Standardized Blood Sampling Protocol
2
HEART Score Troponin Measurement Protocol
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The HEART score only considers admission troponin levels. Troponin levels were measured with the Access AccuTnI+3 Troponin I assay on the UniCel DxI Immunoassay System (Beckmann Coulter, Brea, CA). The cutoff for MI was set at >60 ng/L at the coefficient of variation <10%. The limit of detection was 10 ng/L, and the 99th percentile cut‐off point of 42 ng/L.22
3
Serum 25(OH)D Measurement Protocol
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A total of 2 mL of collected blood was used to measure the levels of serum 25(OH)D which were analyzed using chemiluminescence Unicel DXI immunoassay system (Beckman-coulter, USA) with a detection limit 2.0 ng/mL and a total imprecision ≤ 10.0% CV at concentrations greater than 15.0 ng/mL (37.5 nmol/L) (21 (link)). Moreover, the total Standard Deviation (SD) was ≤ 1.5 ng/mL (3.8 nmol/L) at concentrations ≤ 15.0 ng/mL. Subjects were categorized according to serum 25(OH) D levels using the following cutoff values: Vitamin D deficient for serum 25(OH) D concentration<20 ng/ml, based on the levels at which bone-related symptoms become apparent, and insufficiency as a serum 25(OH) D of (20–30 ng/ml, determined by the level of vitamin D repletion sufficient for optimum bone (22 (link)).
4
Comprehensive Blood Biomarker Analysis
5
Comprehensive Biomarker Profiling Protocol
6
Fasting Blood Analysis and Inflammation
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All blood sampling was performed at the GCRC and analysed at VUMC and VA central laboratories. After blood draw was performed, samples were transported on ice and centrifuged at 3000 rpm for 15 min before being kept frozen at −80°C. Plasma fasting glucose concentrations were analysed using the glucose oxidase method (Glucose Analyzer 2; Beckman Coulter, Brea, CA, USA). Biochemistry measurements were analysed at the VUMC Pathology Laboratory. High‐sensitivity C‐reactive protein (hs‐CRP) concentrations were measured by high‐sensitivity particle‐enhanced turbidimetric UniCel DxI Immunoassay system (Beckman Coulter).
7
Quantifying Myocardial Infarct Size via 3D Echocardiography
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Three-dimensional echocardiography was performed as described before [20 , 24 (link)]. In short, pigs were re-anesthetized according to the same protocol after 72 h. Medial sternotomy was performed and a gel-filled flexible sleeve was placed directly on the apex of the heart. An X3-1 transducer on an iE33 ultrasound device (Philips, Eindhoven, The Netherlands) was used to perform the echocardiogram. Images were analyzed offline using QLab 10.1 (3DQ advanced) analysis software. Due to incomplete capture of the LV, one animal was excluded from the analysis. After 3D-echocardiography, animals were killed by exsanguination under anesthesia. The heart was excised and the LV was cut into 5 equal slices from apex to base. Slices were incubated in 1 % TTC (Sigma-Aldrich Chemicals, Zwijndrecht, the Netherlands) in 37 °C 0.9 %NaCl for 10 min to discriminate between infarct tissue and viable myocardium. After incubation, photographs of the slices were made and the infarct size as a ratio of the left ventricle (LV) was quantified using ImageJ software (NIH, Bethesda, MD, USA). Troponin was measured in plasma isolated from blood drawn after 2 h reperfusion, using a UniCel DxI Immunoassay system (Beckman Coulter, Brea, CA, USA) with a paramagnetic particle, chemiluminescent immunoassay (Beckman Coulter, Brea, CA, USA).
8
Vitamin D Assessment in Participants
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At admission participants had three blood samples taken: one serum separating tube, one fluoride oxalate tube and one potassium EDTA tube. These samples were processed for 25-hydroxyvitamin D, random venous plasma glucose and haemoglobin A1c (HbA1c).
25-hydroxyvitamin D was processed by UniCel DxI Immunoassay System using Beckman Access 25(OH) Vitamin D UniCel DxI Reagent Pack. Serum vitamin D concentration reference ranges used in this study are the National Institute for Health and Care Excellence (NICE) recommended ranges as follows: Less than 25 nmol/L is vitamin D deficiency, 25–50 nmol/L is vitamin D insufficiency and greater than 50 nmol/L is vitamin D sufficiency.26
25-hydroxyvitamin D was processed by UniCel DxI Immunoassay System using Beckman Access 25(OH) Vitamin D UniCel DxI Reagent Pack. Serum vitamin D concentration reference ranges used in this study are the National Institute for Health and Care Excellence (NICE) recommended ranges as follows: Less than 25 nmol/L is vitamin D deficiency, 25–50 nmol/L is vitamin D insufficiency and greater than 50 nmol/L is vitamin D sufficiency.26
9
Blood Biomarker Analysis Protocol
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All blood sampling was performed at the Clinical Research Center and analyzed at VUMC central laboratories. Leptin samples were analyzed at Vanderbilt's Hormonal Lab Core. High molecular-weight adiponectin and interleukin 6 (IL-6) were measured by enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, MN). High-sensitivity C-reactive protein (hsCRP) was measured by high-sensitivity particle-enhanced turbidimetric UniCel DxI Immunoassay system (Beckman Coulter) at the Vanderbilt Clinical laboratory.
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