Log In Sign up
The largest database of trusted experimental protocols

Lc3b 2

Manufactured by Leica
Visit Supplier

The Leica LC3b-II is a versatile and reliable lab equipment product designed for precise optical analysis. It features a high-quality optical system and advanced imaging capabilities to support a wide range of scientific research and testing applications. The core function of the LC3b-II is to provide accurate and detailed visual data for users.

Automatically generated - may contain errors

Lab products found in correlation

Qwin 3, by Leica (2 mentions) Lc3b 2, by Cell Signaling Technology (2 mentions) Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions)

2 protocols using lc3b 2

1

Quantifying Cardiomyocyte Morphology and Autophagy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following cell culture on silicon-based plates in serum-free DMEM for 48 hours, cardiomyocytes or COS7 cells were incubated with anti-α-MHC (Upstate, catalogue 05-716, USA) and LC3b-II (Cell Signaling Technology Inc. catalogue 2775, USA). The samples were then incubated with secondary antibodies conjugated with FITC or Alex (Invitrogen, catalogue A21206, USA) according to the manufacturer's instructions. The surface areas of cardiomyocytes and LC3b-II were determined using an image analysis software (Leica Qwin 3) and were calculated using the mean of 100 to 120 cells from randomly selected fields.
Lin L., Tang C., Xu J., Ye Y., Weng L., Wei W., Ge J., Liu X, & Zou Y. (2014). Mechanical Stress Triggers Cardiomyocyte Autophagy through Angiotensin II Type 1 Receptor-Mediated p38MAP Kinase Independently of Angiotensin II. PLoS ONE, 9(2), e89629.
+ Open protocol
+ Expand
2

Quantifying Cardiomyocyte Autophagy Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following cell culture on silicon‐based plates in serum‐free DMEM for 48 hrs, cardiomyocytes were incubated with anti‐α‐MHC (catalogue 05‐716, Upstate, Millipore Corporation, Billerica, Massachusetts, USA) and LC3b‐II (catalogue 2775; Cell Signaling Technology Inc.). Samples were then incubated with secondary antibodies conjugated with FITC or Alex (catalogue A21206; Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. The surface areas of cardiomyocytes and LC3b‐II were determined using an image analysis software (Leica Qwin 3) and were calculated using the mean of 100 to 120 cells from randomly selected fields.
Lin L., Liu X., Xu J., Weng L., Ren J., Ge J, & Zou Y. (2015). Mas receptor mediates cardioprotection of angiotensin‐(1‐7) against Angiotensin II‐induced cardiomyocyte autophagy and cardiac remodelling through inhibition of oxidative stress. Journal of Cellular and Molecular Medicine, 20(1), 48-57.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!