Rnase free dnase on the column digestion kit

Manufactured by Qiagen

Sourced in United Kingdom

The RNase-free DNase on-the-column digestion kit is a laboratory tool designed to remove genomic DNA contamination from RNA samples during RNA purification. The kit utilizes a DNase enzyme that is specifically formulated to be RNase-free, ensuring the integrity of the RNA is maintained throughout the process.

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Lab products found in correlation

Rneasy mini extraction kit, by Qiagen (2 mentions) Superscript vilo cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Nanodrop 1000, by Thermo Fisher Scientific (1 mentions) Luciferase mrna, by Promega (1 mentions) Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

RNase-free DNase (842 citations) RNase-free DNase kit (281 citations) On-column DNase digestion (141 citations) On-column DNase (24 citations) RNase-free DNase digestion (20 citations) On-column DNase digestion kit (7 citations) On-column RNase-free DNase kit (4 citations) RNase-free DNase digestion kit (4 citations) On column-RNase-free DNAse digestion kit (2 citations) RNase free DNase on-column digestion (2 citations)

2 protocols using rnase free dnase on the column digestion kit

Testis RNA Isolation and cDNA Synthesis

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RNA was isolated from frozen testes using the RNeasy Mini extraction kit with RNase-free DNase on-the-column digestion kit (Qiagen, Crawley, UK) according to the manufacturer’s instructions. Due to changes in testis cell composition and size during normal development or due to the impact of gene deletion/treatment, 5 ng of luciferase mRNA (Promega, UK) was added per testis prior to RNA extraction as an external control to allow for direct comparison between samples^{59 (link)}. RNA was quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) andcDNA was prepared using the SuperScript VILO cDNA synthesis kit (Life Technologies) according to manufacturer’s instructions.

Patel S.H., O’Hara L., Atanassova N., Smith S.E., Curley M.K., Rebourcet D., Darbey A.L., Gannon A.L., Sharpe R.M, & Smith L.B. (2017). Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics. Scientific Reports, 7, 8991.

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Pituitary RNA Extraction and cDNA Synthesis

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RNA was isolated from frozen pituitaries from n = 8 d80 Foxg1^Cre/+; AR^fl/y and control mice using the RNeasy Mini extraction kit with RNase-free DNase on-the-column digestion kit (Qiagen, Crawley, UK) according to the manufacturer’s instructions. RNA was quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Random hexamer primed cDNA was prepared using the SuperScript VILO cDNA synthesis kit (Life Technologies) according to manufacturer’s instructions.

O’Hara L., Curley M., Tedim Ferreira M., Cruickshanks L., Milne L, & Smith L.B. (2015). Pituitary Androgen Receptor Signalling Regulates Prolactin but Not Gonadotrophins in the Male Mouse. PLoS ONE, 10(3), e0121657.

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