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Human bdnf quantikine elisa

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The Human BDNF Quantikine ELISA is a quantitative sandwich enzyme immunoassay designed for the measurement of human brain-derived neurotrophic factor (BDNF) concentrations in cell culture supernates, serum, and plasma.

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Quantikine® ELISA Human BDNF Immunoassay Human Free BDNF Quantikine ELISA Kit Quantikine® ELISA Human Free BDNF Immunoassay Human BDNF Quantikine ELISA kit Quantikine ELISA Quantikine Human Quantikines ELISAs

2 protocols using human bdnf quantikine elisa

1

Quantifying Serum BDNF Levels via ELISA

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Levels of serum BDNF were quantified using a sandwich enzyme-linked immunosorbent assay for Human Free BDNF (Human BDNF Quantikine ELISA, R&D Systems, Minneapolis, MN) in accordance with the manufacturer’s instructions and our standard protocol (Bharani et al., 2017 (link), 2020 (link); Håkansson et al., 2017 (link)). This assay has an intra-assay and inter-assay variability of 5 and 9% respectively, and a minimum sensitivity of 20 pg/ml. Serum samples were diluted 20-fold in the dilution buffer provided in the assay kit and BDNF concentrations were determined via non-linear regression from the standard curves using GraphPad Prism version 8 (GraphPad Software, San Diego, CA).
Grossberg A.N., Bettcher B.M., Gorgens K.A, & Ledreux A. (2021). Curiosity-Based Interventions Increase Everyday Functioning Score But Not Serum BDNF Levels in a Cohort of Healthy Older Adults. Frontiers in Aging, 2, 700838.
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2

Altitude-Induced Hypoxia and BDNF Levels

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Under a Mayo Clinic Institutional Review Board-approved study related to altitude sickness, blood samples from 30 healthy volunteers were collected as part of a protocol assessing physiological activity and sleep under conditions of normoxia (21% O2; McMurdo Station, Antarctica; elevation 112 ft) vs. hypoxia (~15% O2; morning after 2nd night at altitude at the South Pole Station; elevation ~11,000 ft, alveolar PO2=100mmHg; average O2 saturation 88%). Studies conformed to the rules and regulations of the Declaration of Helsinki. Blood samples were centrifuged, supernatant (blood serum) transferred to cryovials and stored at −80°C. Serum samples were analyzed for BDNF levels using a human BDNF Quantikine ELISA (R&D Systems, Minneapolis).
Helan M., Aravamudan B., Hartman W.R., Thompson M.A., Johnson B.D., Pabelick C.M, & Prakash Y.S. (2014). BDNF Secretion by Human Pulmonary Artery Endothelial Cells in Response to Hypoxia. Journal of molecular and cellular cardiology, 68, 89-97.
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