The cells were lysed with RIPA buffer (Thermo Fisher Scientific) to prepare total cell lysates. Ten micrograms of each cell lysate were loaded on SDS-PAGE gels prior to immunoblotting. The antibodies used for immunoblotting were: IFNλ4 (1:200, mouse, Millipore MABF227), IFNλ4 (1:200, rabbit, Abcam ab196984), STAT1 (1:1000, rabbit, BD Biosciences 610120), PY-STAT1 (1:1000, mouse, BD Biosciences 612233), STAT2 (1:1000, rabbit, Santa Cruz Biotechnology sc-476), IRF9 (1:1000, rabbit, Santa Cruz sc-496), SOCS1 (Abcam #62584), USP18 (Cell Signaling Technology #4813), horseradish peroxidase (HRP)-conjugated rabbit IgG (1:5000, Abcam ab97051), and HRP-conjugated mouse IgG (1:5000, Abcam ab97023).
Py stat1
Manufactured by BD
The PY-STAT1 is a laboratory equipment product from BD. It is designed to perform statistical analysis on data collected from various laboratory experiments and processes.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (4 mentions)
Mabf227, by Abcam (2 mentions)
Hrp conjugated rabbit igg, by Abcam (2 mentions)
Hrp conjugated mouse igg, by Abcam (2 mentions)
Horseradish peroxidase, by Abcam (1 mentions)
Stat2, by Santa Cruz Biotechnology (1 mentions)
Usp18, by Cell Signaling Technology (1 mentions)
Stat1, by BD (1 mentions)
T6074, by Merck Group (1 mentions)
Anti flag m2 affinity gel, by Merck Group (1 mentions)
2 protocols using py stat1
1
Immunoblotting Analysis of IFNλ4 Signaling
2
Immunoblotting of IFN-λ4 Signaling Pathway
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To prepare total cell lysates, the cells were lysed with RIPA buffer (Thermo Fisher Scientific). Ten micrograms of each cell lysate were loaded on SDS-PAGE gels. The antibodies used for immunoblotting were as follows: IFN-λ4 (1:200, mouse, Millipore MABF227), IFN-λ4 (1:200, rabbit, Abcam ab196984), FLAG (1:500, mouse, Sigma, F3165), STAT1 (1:1000, rabbit, BD Biosciences 610120), PY-STAT1 (1:1000, mouse, BD Biosciences 612233), STAT2 (1:1000, rabbit, Santa Cruz Biotechnology sc-476), IRF9 (1:1000, rabbit, Santa Cruz sc-496), ISG15 (1:1000, mouse, Santa Cruz Biotechnology sc-69701), USP18 (1:1000, rabbit, Cell Signaling 4813S), HCV core (1:1000, mouse, Thermo MA1-080), tubulin (mouse, Sigma T6074), horseradish peroxidase (HRP)-conjugated rabbit IgG (1:5000, Abcam ab97051), HRP-conjugated mouse IgG (1:5000, Abcam ab97023). For immunoprecipitation of FLAG-tagged IFN-λ4 protein, anti-FLAG M2 Affinity Gel (Sigma) was used.
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