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4 protocols using sulfameter

1

Quantitative Bioanalytical Workflow

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LC-MS grade acetonitrile, methanol, and water were purchased from J.T. Baker (Milwaukee, WI, USA). Amlodipine, atenolol, atorvastatin, digoxin, enalapril, losartan, propranolol, simvastatin, and sulfameter were purchased from Sigma–Aldrich (St. Louis, MO, USA). Fenofibrate, furosemide, nifedipine, and valsartan were purchased from Tokyo Chemical Industry (Tokyo, Japan). Whatman 903 blood sampling cards were purchased from GE Healthcare (Westborough, MA, USA). Human whole blood with EDTA was purchased from Innovative Research (Novi, MI, USA), which is a supplier of human blood samples. Experimental usage of human blood was approved by the Institutional Review Board of Seoul National University (IRB NO. E1605/003-003).
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2

Analytical Method for Pharmaceuticals

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Acetaminophen (ACT), acetylsulfamethoxazole (ASMX), azithromycin (AZI), caffeine (CAF), carbamazepine (CBZ), clarithromycin (CLA), enrofloxacin (ENRO), erythromycin (ERY), iopamidol (IOPA), norfloxacin (NOR), oxytetracycline (OTC), sarafloxacin (SARA), sulfachloropyridazine (SCP), sulfadiazine (SPD), sulfadimethoxine (SDM), sulfamerazine (SMR), sulfameter (SMT), sulfamethazine (SMZ), sulfamethizole (SMI), sulfamethoxazole (SMX), tetracycline (TC), and trimethoprim (TMP) were purchased from Sigma-Aldrich. Ciprofloxacin (CIP) and diclofenac (DIC) were obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). Sulfathiazole was purchased from ICN Biomedicals, Inc. (Irvine, CA). carbamazepine-d10 (d10-CBZ) was purchased from CDN Isotopes (Quebec, Canada). Chlortetracycline (CTC) was obtained from Acros Organics (VWR International, Westchester, PA). Paroxetine maleate (PRX) and venlafaxine (VEN) were obtained from Cerilliant (Sigma-Aldrich, St Louis, MO). The Barnstead NANOpure™ DIamond (Waltham, MA) purification system was used to obtain 18.2 MΩ water. LC-MS grade methanol and acetonitrile were obtained from EMD Millipore Corporation (Billerica, MA), and formic acid (88%) was purchased from Fisher Chemical (Pittsburgh, PA). Oasis™ HLB solid-phase extraction (SPE) cartridges were purchased from Waters (Milford, MA).
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3

Sulfamethazine-specific Aptamer Screening

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Sulfamethazine-specific aptamer, SMZ1S (5′-CGTTAGACG-3′), was screened in our laboratory.23 (link) HAuCl4·4H2O, SMZ, sulfadimethoxine, sulfameter and nitrofurantoin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sulfapyridine, ofloxacin, chlortetracycline, doxycycline and chloramphenicol were purchased from Aladdin Biotechnology Inc. (Shanghai, China). Rhodamine B was obtained from Sangon Biotechnology Inc (Shanghai, China). Unless otherwise mentioned, all other reagents were of analytical grade. Ultrapure water was used for all experiments and obtained using a Millipore Milli-Q system (Millipore, Bedford, MA, USA).
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4

Aptamer-based Detection of Antibiotics

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SQX-specific aptamers have been previously screened by our research group [4 (link)]. SQX, sulfameter (SME), sulfamethazine (SMZ), sulfadimethoxine (SDM), sulfamonomethoxine (SMM), HAuCl4·4H2O, and graphene oxide were obtained from Sigma-Aldrich (St. Louis, MO, USA). Chlortetracycline (CTC), oxytetracycline (OTC), ofloxacin (OFL), and chloramphenicol (CAP) were obtained from Aladdin Biotechnology Inc. (Shanghai, China). RhoB and oligonucleotides were obtained from Sangon Biotechnology Inc. (Shanghai, China). Deionized water was purified using Millipore Milli-Q Ultrapure Water System (Bedford, MA, USA) and used for all experiments. DNA oligonucleotide stock solutions were prepared with binding buffer. All other chemicals and reagents were of analytical grade and obtained from Beijing Chemical Reagent Company (Beijing, China).
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