Retiga r1 camera

For morphological studies, Sf9 cells were cultured in 96-well plates at the same density used for viability experiments, in the presence of the molecules under study. After incubation, cells were washed with Hanks’ balanced salt solution (HBSS) and fixed in 10% formalin solution for 30 min, at room temperature. CF543 (5 U/mL) and DAPI (0.25 µg/mL) were added, and cells were stained for 25 min at room temperature and washed with HBSS.
Images were acquired in an inverted Eclipse Ts2R-FL (Nikon) equipped with a Retiga R1 camera and an S Plan Fluor ELWD 20x DIC N1 objective. Images were analyzed with Fiji [38 (link)]. For quantitative parameters, the Fiji’s Cell Counter plugin was used.

The cells were cultured in 96-well plates at a density indicated above and treated with the compounds under study. The cells were incubated for 24 h, after which they were washed with HBSS and fixed with a 10% formalin solution for 20 min at room temperature. Phalloidin–CF543 (5 U/mL) and DAPI (0.25 µg/mL) were added, and the cells were stained for 30 min at room temperature.
Images were acquired in an inverted Eclipse Ts2R-FL (Nikon) equipped with a Retiga R1 camera and treated with Fiji.