Cultured cells were lysed by using lysis buffer supplemented with protease and phosphatase inhibitors9 (link). Cellular proteins (20 or 100 μg) were separated by SDS-PAGE. The following Ab were used at dilutions recommended by manufacturers: anti-GNMT Ab (YMAC Biotech Co, Taipei, Taiwan), anti-FLAG Ab, anti-β-actin and anti-α-tubulin Ab (Sigma). Immunoblotting signals were normalized to anti-β-actin or anti-α-tubulin and quantified by densitometric scanning.
Anti α tubulin ab
Manufactured by Merck Group
Sourced in United States
Anti-α-tubulin Ab is a laboratory reagent used for the detection and analysis of α-tubulin, a structural protein found in the cytoskeleton of eukaryotic cells. This antibody can be used in various experimental techniques, such as immunohistochemistry, immunocytochemistry, and Western blotting, to identify and study the expression and distribution of α-tubulin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (1 mentions)
Anti flag ab, by Merck Group (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti p foxo3a, by Cell Signaling Technology (1 mentions)
Anti bim, by Cell Signaling Technology (1 mentions)
Anti bcl xl, by Cell Signaling Technology (1 mentions)
Anti parp, by Cell Signaling Technology (1 mentions)
Fluorescent mounting medium, by Agilent Technologies (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Carl Roth (1 mentions)
Affinipure donkey anti mouse antibody, by Jackson ImmunoResearch (1 mentions)
3 protocols using anti α tubulin ab
1
Western Blot Analysis of Cellular Proteins
2
Western Blot Analysis of Apoptosis Regulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot analyses were performed according to the standard method as described previously (23 (link)), using anti-SphK1 antibody (Abgent, San Diego, CA, USA); anti- Akt, anti-p-Akt, anti-cleaved caspase 3, anti-PARP, anti-Bim, anti-bax, anti-Bcl-xL, anti-p-FoxO3a, and anti-Bcl-2 antibodies (Cell Signaling, Danvers, MA, USA). The membranes were stripped and re-blotted with an anti-α-tubulin Ab (Sigma, St. Louis, MO, USA) as a loading control.
3
Tubulin Polymerization Assay in A431 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For tubulin polymerization experiments, A431 cells (2.5 × 10 5 cells per well) were grown overnight on (3-aminopropyl)triethoxysilane coated coverslips in 24-well plates and subsequently incubated with 100 nM PTX, dPGS amine (1), dPGS-PTX (3), dPG-PTX (4) for 16 h. After incubation, cells were rinsed with PBS, fixated with 4% formaldehyde for 8 min, and stored overnight in 0.1% formaldehyde. After rinsing, the cells were permeabilized with 0.5% Triton X-100/PBS (4 min) and blocked with a sterile filtered Milk/PBS-solution. Primary monoclonal anti-α-tubulin-AB (Sigma-Aldrich. 1 : 4000 in dry milk) was incubated for 1 h at rt, followed by rinsing and incubation of secondary Cy™3-conjugated AffiniPure donkey antimouse antibody (Jackson ImmunoResearch. 1 : 200 in Milk; 1 h at rt). After nuclear counterstaining with 4′,6-diamidino-2phenylindole (DAPI; Carl Roth. 1 : 500 in PBS, 15 min), the slides were mounted in fluorescent mounting medium (Dako).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!