Anti mouse cd31 antibody

Manufactured by Dianova

Sourced in Germany

The Anti-mouse CD31 antibody is a primary antibody used in immunohistochemistry and flow cytometry applications to detect the CD31 protein, also known as PECAM-1, which is expressed on the surface of endothelial cells. This antibody can be used to identify and analyze endothelial cells in mouse tissue samples.

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Lab products found in correlation

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Close spelling variants of this product

Rabbit anti-mouse CD31 antibody Rat anti-mouse CD31 antibody Mouse-anti-CD31 Anti-CD31 antibody CD31 antibody Anti-CD31

3 protocols using anti mouse cd31 antibody

Tissue Staining and Immunohistochemical Analysis

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The paraffin section was stained with hematoxylin and eosin (HE), Masson’s trichrome (MT) and Picrosirius red (PSR) [17 (link),23 (link)]. In immunohistochemical analysis, GFP antibody (Abcam plc, Tokyo, Japan), anti-mouse αSMA antibody (Abcam plc, Tokyo, Japan), anti-mouse CD31 antibody (Dianova GmbH, Hamburg, Germany), anti-mouse neutrophil antibody (NIME-R14: Abcam plc, Tokyo, Japan) and anti-mouse TGF-β1 antibody (Abcam plc, Tokyo, Japan). An EnVision Kit (DAKO Japan Inc., Tokyo, Japan) was used for visualization. Semi-quantitative evaluation was performed: negative (Grade 0), slightly positive (Grade 0.5), positive (Grade 1) and strongly positive (Grade 2).

Jimi S., Kimura M., De Francesco F., Riccio M., Hara S, & Ohjimi H. (2017). Acceleration Mechanisms of Skin Wound Healing by Autologous Micrograft in Mice. International Journal of Molecular Sciences, 18(8), 1675.

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Evaluating Tumor Vascular Density and Cell Death

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After MSOT acquisition of the last assigned time point (1, 24 or 48 h post WST11-VTP treatment) the mice were sacrificed by carbon dioxide asphyxiation. The subcutaneous tumors were subsequently necropsied, including the skin layer on the surface marked along the imaging plane to ensure comparable orientation, and fixed in 10% neutral buffered formalin. They were cut and paraffin embedded keeping the original orientation and previous imaging plane. The sections were stained with hematoxylin-eosin (H&E), and for immunohistochemistry analysis the tumor samples were stained with anti-mouse CD31 antibody (Dianova, Hamburg, Germany) to assess tumor vessel density and terminal deoxynucleotidyl transferease dUTP nick-end labeling (TUNEL) for cell death.

Neuschmelting V., Kim K., Malekzadeh-Najafabadi J., Jebiwott S., Prakash J., Scherz A., Coleman J.A., Kircher M.F, & Ntziachristos V. (2018). WST11 Vascular Targeted Photodynamic Therapy Effect Monitoring by Multispectral Optoacoustic Tomography (MSOT) in Mice. Theranostics, 8(3), 723-734.

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Histopathological Analysis of Tumor Toxicity

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Tumors, kidneys and pancreas were fixed in 10% buffered formalin (Fisher Scientific, Pittsburgh, PA), embedded in paraffin, sectioned at 5 micron thickness, and stained with hematoxylin-eosin (H&E). Histopathological analysis to assess tissue toxicity induced by different treatment regimens was performed by a board-certified veterinary pathologist. For immunohistochemistry (IHC) analysis of tumor, samples were stained with anti-mouse CD31 antibody (Dianova, Hamburg, Germany) to assess tumor vessel density and terminal deoxynucleotidyl transferease dUTP nick-end labeling (TUNEL) for cell death (19 (link)).

Kim K., Zhang H., La Rosa S., Jebiwott S., Desai P., Kimm S., Scherz A., O’Donoghue J.A., Weber W.A, & Coleman J.A. (2017). Bombesin Antagonist Based Radiotherapy of Prostate Cancer Combined with WST-11 Vascular Targeted Photodynamic Therapy. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(13), 3343-3351.

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