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52 protocols using milli q grade

1

Photoelectrochemical Analysis of Pharmaceutical Compounds

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2.1 Chemicals for Analytical and Photoelectrochemical experiments: Levofloxacin (Fluka, purity >98%), ketoprofen (Sigma-Aldrich, purity >98%), sodium sulfate (Sigma-Aldrich, purity, 99%), sulfuric acid (Sigma-Aldrich, 95%), 1 M phosphate buffer (pH 7.4, Sigma-Aldrich) and 0.5 M borate buffer (pH 9, Alfa), Na2SO4 (Fluka, 99%), K2IrCl6 (33.4 % Ir, Alfa Aesar), sodium acetate (Riedel de Hӓen 99 %), 70 % HNO3 (Sigma-Aldrich), V2O5 (Alfa Aesar 99.6 %), VOSO4 × x H2O (Alfa Aesar, 99.9 %), Bi(NO3)3 × 5 H2O (Alfa Aesar 99.99 %), methanol, formic acid, sodium hydroxide, sodium citrate, High-Performance Liquid Chromatography (HPLC) grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany). The water was Milli-Q® grade (Millipore, MA, USA).
High-performance liquid chromatography (HPLC) grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany). The water was Milli-Q® grade (Millipore, MA, USA).
When necessary, a dilute aqueous solution of sulfuric acid was employed to adjust at 6 the pH of the drug solution (10 ppm Levofloxacin or ketoprofen) containing 0.7mM of sodium sulfate as supporting electrolyte. The saline concentration was chosen to reproduce the average salinity of freshwater. 28 The pH of the drug solutions was measured with a combined glass electrode connected to an AMEL pH-meter (Milano, Italy).
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2

Synthesis and Characterization of Multifunctional Nanoparticles

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Chloroauric acid (HAuCl4·3H2O), sodium borohydride (NaBH4), cetyltrimethyl ammonium bromide (CTAB), silver nitrate (AgNO3), tetraethylorthsilicate (TEOS), 3-(Methacryloxy) propyl triethoxysilane (MPS), N-isopropylacrylamide (NIPAM), N,N’-Methylenebis (acrylamide) (MBA), sodium dodecyl sulfate (SDS), potassium persulfate (KPS), poly(vinylpyrrolidone) (PVP, 24,000) were purchased from Sigma-Aldrich. Chlorine E6 (ICG) was purchased from MedKoo Biosciences. The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories. Deionized water (Millipore Milli-Q grade) with resistivity of 18.2 MΩ was used in all the experiments.
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3

Lipidomic Extraction Protocol

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Methanol, acetonitrile, chloroform, and ethanol were from Fisher Chemicals. Formic acid and DMSO were from Sigma-Aldrich, Gillingham UK. All solvents were HPLC-grade, apart from Formic acid, which was UPLC-grade. Water used was Milli-Q grade (Milipore, UK). Sphingolipid internal standards were obtained from Matreya LLC (State College, PA, USA): 13C6 glucosyl-sphingosine, N-glycinated lyso-ceramide trihexoside, N-omega-CD3-hexadecanoyl-glucopsychosine, N-omega-CD3-hexadecanoyl-lactosylceramide, and N-omega-CD3-octadecanoyl-ceramide trihexoside further details are available in Supplementary Table S2.
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4

Synthesis and Characterization of Cobalt Ferrite Nanoparticles

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Cobalt chloride (II) hexahydrate (ACS reagent, 98%), iron chloride (III) hexahydrate (ACS reagent, 97%), oleic acid (90%), hexane (ACS reagent, ≥98.5%), diethyl ether (anhydrous, ACS reagent, ≥99%), toluene (ACS reagent, ≥99.5%), and chloroform (for high-performance liquid chromatography, gas chromatography, and residue analysis, ≥99.9%) were purchased from Sigma-Aldrich. 1-Octadecene (90%) was purchased from Acros Organics, and sodium oleate (>97%) was purchased from TCI America. PEG, 5 kDa for ligand exchange, was procured from Sigma-Aldrich, and block copolymer polyethylene glycol-b-poly(lactic acid) PEG4.9kDa-b-PLA6.0kDa was purchased from Evonik Industries. Tetrahydrofuran (THF; 99.9% Acros Organics) was dried using sodium sulfate before use in Flash Nanoprecipitation (FNP). HA sodium salt from Streptococcus equi was purchased from Sigma-Aldrich. Bovine synovial fluid was obtained from Animal Technologies. Water was used from Milli-Q grade (Millipore, Bedford, MA).
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5

Thyme Essential Oil Chitosan Nanocarriers

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Thyme essential oil (ρ = 0.917 g/cm3 at 25 °C) from Sigma-Aldrich® (St. Louis, MO, USA) (Lot: MKCF4333) is used to form the oil nucleus. Chitosan (MW = 50–190 kDa, μ = 20–300 cP in CH3COOH solution (1 g/L) at 25 °C) (Lot: STBH6262) as the encapsulating polymer, Tween® 80 and Span® 80 (Lot: MKBV4425V) were purchased from Sigma Aldrich®. The distilled water was Milli-Q® grade (Millipore Corporation, Bedford, MA, USA). ABTS (2,2′-azino-bis- (3-ethylbenzthiazoline)-6-sulfonic acid) (Lot: SLBD8908V), DPPH (2,2-diphenyl-1-picrylhydracil) (Lot: STBG9431), FRAP (2,4,6-tri (2-pyridyl)-s-triazine) (Lot: BCBW6989) were purchased from Sigma Aldrich® (St. Louis, MO, USA). Ascorbic acid (C6H8O6) (Lot: A0616289), potassium persulfate (K2S2O8) (Lot: M1010511), potassium dihydrogen phosphate (KH2PO4) (Lot: M0918575), dipotassium hydrogen phosphate (KH2PO4) (Lot: M1014575), and glacial acetic acid (CH3-COOH) (Lot: A0414235) were purchased from Meyer® (Reactivos Química Meyer, Ciudad de México, México). Cyclohexane (C6H12) and methanol (CH3OH) (Lot: 824333) were purchased from Fermont® (Productos Químicos Monterrey, México).
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6

Synthesis and Characterization of MWCNT Nanomaterials

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Chemicals including carboxylic MWCNTs (outer
diameter: 8–15 nm, length: 50 μm, 95%), 4-hydroxyaminobenzene
(98%), phenol (99%), diethylenetriamine (99%), SDS (99%), and 1,2-dibromoethane
(99%) were obtained from J&K Chemicals. 11-Bromoundecanoic acid
(95%) and PEI with a molecular weight of 10 000 were purchased
from Aladdin Industrial Corporation. PPE membrane (mean pore size
0.22 mm) was obtained from Xiya Reagent. Ferrocene (98%) and all other
analytical grade reagents including methanol, dichloromethane, hydrochloric
acid, sodium hydroxide, and FeCl3 were obtained from Sinopharm
Chemical Reagent Co, Ltd. All chemicals were used as received without
further purification. The surfactant FADETA was synthesized according
to the procedure as mentioned in Scheme S1 in the Supporting Information. Water was Millipore Milli-Q-grade.
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7

Tyrosine Derivatives and Oxidative Stress Analysis

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Acetonitrile (LC-MS grade), methanol (LC-MS grade) and formic acid (analytical grade) were purchased from Sigma Aldrich Quimica SA (Madrid, Spain). Water was Milli-Q grade (>18.2 MΩ) from a Millipore purification system. Standards of o-Tyr, m-Tyr, Phe, 3NO2-Tyr, 3Cl-Tyr, p-Tyr, 8OHdG and 2dG (>96% w/w purity) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Deuterated phenylalanine (Phe-D5) was purchased from CDN Isotopes (Pointe-Claire, Canada).
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8

Synthesis of Gold Nanoparticles

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Cetyltrimethylammonium bromide (CTAB), H2SO4, choloroauric acid, NaBH4, AgNO3, L-ascorbic acid (AA) and NaOH were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Thiolated polyethylene glycol (PEG, molecular weight 5000) was purchased from Shanghai Yare Biotech, Inc (Shanghai, China). The RGD peptides were purchased from China Peptides Co., Ltd (Shanghai, China). Deionized water (Millipore Milli-Q grade) with a resistivity of 18.2 MΩ was used in all experiments.
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9

Synthesis of Epoxide Compounds

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Carbon papers (TGP-H-090) were purchased from Toray Industries, Inc (Tokyo, Japan). Potassium bromide (KBr, 99%), potassium chloride (KCl, 99.5%), potassium iodide (KI, 99%), sodium hydroxide (NaOH, 96%), nitric acid (HNO3, 65-68%), silver nitrate (AgNO3, 99.8%) and hydrochloric acid (HCl, 36-38%) were all purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). PO (99.7%), propylene bromohydrin (75%), ethylene oxide (99.5%), 1,2-butylene oxide (99%), isobutylene oxide (97%), 1,2-epoxyoctane (97%), cyclopentene oxide (97%), and styrene oxide (97%) were purchased from Aladdin Co. Ltd. (Shanghai, China). 4,4-dimethyl-4-silapentane−1-sulfonic acid (DSS), dimethyl sulfoxide-d6 (DMSO-d6, 99.9 atom% D), and Nafion 115 membrane were purchased from Sigma-Aldrich. The deionized (DI) water with a resistivity of 18.2 MΩ cm was provided by a Millipore Milli-Q grade. All of the chemicals were used without any further purification.
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10

Enzymatic Assays of Bioactive Compounds

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Tyrosinase from mushroom, L-3,4-dihydroxyphenylalanine (L-DOPA), elastase from porcine pancreas, N Succinyl-Ala-Ala-Ala-p-nitroanilide (AAAPVN), Acetylcholinesterase (AChE) from Electrophorus electricus, acetylthiocholine iodide, DTNB (5,5′-dithiobis (2-nitrobenzoic acid), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-Tripyridyl-s-Triazine (TPTZ), Tris(hydroxymethyl)aminomethane, hydrochloric acid (36%), Trolox, Folin Ciocaltue’s reagent, gallic acid (95%), rutin (95%), quercetin (97%) and neostigmine bromide were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Kojic acid (Alfa Aesar™, 99%), sodium phosphate monobasic monohydrate (ACROS Organics™, 98+%) and sodium hydrogen phosphate (Alfa Aesar™, 98+%) were provided by Fisher Scientific (Hampton, VA, USA). Ethanol absolute was purchased from Sodipro (Echirolles, France). All other chemicals and reagents used were analytical grade. The water used for the extraction and preparation of water-based solutions was Milli-Q grade (Millipore, Burlington, MA, USA).
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