Dri chem 7000

Blood samples from fasting rats were collected from the cava vein at sacrifice. Glucose, bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides, and albumin were measured with standard methods at the Hospital Vall d’Hebron CORE lab. Insulin was measured using an ELISA kit following the manufacturer’s instructions (EMD Millipore, Billerica, MA, USA). Insulin resistance was estimated by applying the homeostasis model of insulin resistance index (HOMA-IR): (fasting insulin (ng/mL) × fasting glucose (mg/dL))/405.
In the STAM™ mice, non-fasting blood was drawn from the facial vein for the quantification of glycated hemoglobin (HbA1c), cytokeratin (CK)-18, and biochemistry. HbA1c levels were quantified in whole blood by DCN2000 + (Siemens Healthcare Diagnostics, USA). Serum CK-18 levels were measured by using the Mouse Cytokeratin 18-M30 ELISA kit (Cusabio Biotech Co., Ltd., Wuhan, China). Serum alanine aminotransferase (ALT) and triglyceride levels were measured by FUJI DRI-CHEM 7000 (Fujifilm Corporation, Tokyo, Japan). Serum total cholesterol, high-density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL)-cholesterol levels were quantified by HPLC at Skylight Biotech Inc. (Akita, Japan).

Blood samples obtained from the euthanised marmosets (case nos. 1, 3, 4, 6–14) were used. Complete blood count was analysed using a Sysmex XT-2000i (Sysmex Corporation, Kobe, Japan). The clinical chemistry was analysed using DRI-CHEM 7000 (Fujifilm Corporation, Tokyo, Japan).

Wild type C57BL/6J male mice (6–8 weeks) were purchased from Xuzhou Medical University Animal Center and maintained under standard housing conditions. All animal procedures and protocols are approved by the Experimental Animal Ethics Committee of Xuzhou Medical University, and implemented in accordance with the Xuzhou Medical University Laboratory Animal Care and Use Guide.
The mice of each group (n = 4) were irradiated with signal-dose (5 Gy) at the right thigh. After 24 h, the mice were continuously injected via tail vein with abPTX (equivalent 2.7 mg/kg PTX) or NP-abPTX (equivalent 2.7 mg/kg PTX) for five days. Then, the whole blood samples from mice were collected for hematological analysis using Mindray hematologic analyzer (BC-5300, Mindray Bio-Medical Electronics, Shenzhen, China). For liver and kidney function analysis, the blood urea nitrogen (BUN), creatinine (Cre), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured by DRI-CHEM 7000 (FUJI, Tokyo, Japan).

Blood samples from 16-week-old mice were collected from the inferior vena cava under 4 % isoflurane anesthesia. Hemoglobin concentration (Hb), hematocrit (Ht), red blood cell count (RBC) and blood urea nitrogen (BUN) were measured using an automated counter (KX-21NV; Sysmex Corporation, Kobe, Japan) and an automated biochemical analyzer (Dri-Chem 7000; Fuji Photo Film, Tokyo, Japan) as described previously [15 (link)].