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Exoglow vivo ev labeling kit near ir

Manufactured by System Biosciences
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Sourced in United States

The ExoGlow™-Vivo EV Labeling Kit (Near IR) is a product designed for labeling extracellular vesicles (EVs) with a near-infrared fluorescent dye. The kit provides a method for efficient labeling of EVs, enabling their detection and tracking in various applications.

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Lab products found in correlation

In vivo imaging system ivis, by PerkinElmer (1 mentions)

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ExoGlow-Vivo EV Labeling Kit

2 protocols using exoglow vivo ev labeling kit near ir

1

Near-IR Fluorescent Labeling of EVs

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IMRCs -EV were labeled with a near-infrared fluorescent dye. According to the manufacturer’s instructions for the ExoGlow™-Vivo EV Labeling Kit (Near IR) (EXOGV900A-1, System Biosciences, LLC., USA), 500 μl EV suspension containing approximately 250 μg protein equivalent (determined by BCA test) was incubated with NIR dye at room temperature for 45 min. Labeled EVs were recovered with 167 μl ExoQuick-TC. After incubation, labeled EVs were centrifuged at 13,000 g for 10 min to remove excess dye and resuspended in PBS buffer for in vivo imaging.
Yang S., Liu P., Gao T., Song D., Zhao X., Li Y., Wu J., Wang L., Wang Z., Hao J., Wang C, & Dai H. (2022). Every road leads to Rome: therapeutic effect and mechanism of the extracellular vesicles of human embryonic stem cell-derived immune and matrix regulatory cells administered to mouse models of pulmonary fibrosis through different routes. Stem Cell Research & Therapy, 13, 163.
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2

In Vivo Tracking of Labeled Extracellular Vesicles

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3D-EVs were labeled fluorescently with ExoGlow-Vivo EV Labeling Kit (Near IR) (Cat #EXOGV900A-1, System Biosciences) according to the individual manufacturer’s instructions. The labeled EVs (3 × 1010 EVs/mouse) were administered intravenously via the tail vein in PT stroke and normal mice. Animals were imaged at various time points (3, 24, and 72 h) using IVIS In Vivo Imaging System (PerkinElmer, Waltham) and continuously observed the biodistribution for up to 72 h. Data are expressed as photons/s/cm2/sr (sr: steradian).
Son J.P., Kim E.H., Shin E.K., Kim D.H., Sung J.H., Oh M.J., Cha J.M., Chopp M, & Bang O.Y. (2023). Mesenchymal Stem Cell-Extracellular Vesicle Therapy for Stroke: Scalable Production and Imaging Biomarker Studies. Stem Cells Translational Medicine, 12(7), 459-473.
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