Control igg sc 2025

Primary antibodies used included rabbit anti-Ras (#3965, Cell Signaling Technologies, 1:1000), anti-pSTAT3 Y705 (#9145 S, Cell Signaling Technologies, dilution 1:1000), anti-pSTAT3 S727 (#9134D, Cell Signaling Technologies, dilution 1:1000), anti-STAT3 (#4904 S, Cell Signaling Technologies, dilution 1:1000), mouse anti-SV40-T antigens (MABF121, EMD Millipore, dilution 1:1000), and mouse anti-β-actin (A5441, Sigma-Aldrich, 1:5000). For IL-6 signaling neutralization studies, anti-IL-6R antibody (MAB227, R&D Systems) or control IgG (sc-2025, Santa Cruz Biotechnology) at 2 µg/ml concentration was added to CM for 1 h prior to adding CM to epithelial cells. Cells were treated with CM for 2 h prior to harvesting cells for western blotting. Anti-mouse (#7076, dilution 1:10000), and anti-rabbit (#7074, dilution 1:10000) horseradish peroxidase (HRP) linked secondary antibodies were purchased from Cell Signaling Technologies. Cell lysates were prepared in radioimmunoassay buffer and analyzed by western blotting^{43 (link)}. Uncropped blots are presented in the Source data file.

Anti-MFG-E8 (sc-271574; 1:200 dilution for immunoblotting, immunofluorescence, and immunohistochemistry; 2 µg per 1 mg of total protein for immunoprecipitation), anti-USP14 (sc-398009, 1:200 for immunoblotting, 2 µg per 1 mg of total protein for immunoprecipitation), and anti-GPx4 (sc-166570, 1:400 for immunoblotting) antibodies were purchased from Santa Cruz Biotechnology, USA. Anti-SLC7A11 (12691, 1:1000 for immunoblotting) and anti-Ub (3936, 1:1000) were from Cell Signaling Technology, USA. Anti-Flag (F7425, 1:1000) was from Sigma-Aldrich, USA. Antibodies against β-actin (60008–1-Ig, 1:5000), β-tubulin (10068-1-AP, 1:1000), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (60004-1-Ig, 1:1000) were from Proteintech, China. Anti-USP14 (AF301319, 1:100 for immunofluorescence) was from AiFang Biological, China. Anti-GPx4 (ab125066, Abcam, UK, 1:100), and anti-SLC7A11 (26864-1-AP, Proteintech, China, 1:50) antibodies were used for immunohistochemistry (IHC) staining. Immobilized protein A/G beads (45350) were purchased from Thermo Fisher Scientific, USA. Control IgG (sc-2025) came from Santa Cruz Biotechnology, USA. Cycloheximide (CHX) (S7418), RSL3 (S8155), and Ferrostatin-1 (Fer-1) (S7243) were from Selleck, China. MG132 (HY-13259) was purchased from MedChemExpress, USA.

The anti-Fra-1- (sc-376148X and sc-28310X),—Pol II- (sc-55492X) and -GAPDH (sc-25778) antibodies were from Santa Cruz Biotechnology, as well as control IgG (sc-2025). The anti-p300- (D2X6N, #54062) -CBP (D6C5, #7389) and -CDK7 (#2916) antibodies were from Cell Signaling Technology. The anti-Pol II-PSer5 antibody (clone H14, # 920304) was from Biolegend. The mouse anti-IgM antibody (04–6800) was from Invitrogen. The anti-H3K36me3 antibody (61101) was from Active Motif and the anti-H3 antibody (05–928) from Millipore. The anti-H3K27ac (Ab4729) and -p300/CBP antibodies (Ab14984) were from Abcam. The anti-rabbit (sc-2313) and anti-mouse (sc-2954) horseradish peroxidase (HRP)-conjugated secondary antibodies were from Santa Cruz Biotechnology.