DNA fragments were synthesized as IDT gBlocks Gene Fragments and/or oligonucleotides and amplified by PCR using Q5 Hot Start (New England Biolabs), and cloned into an AAV expression vector (Addgene plasmid # 20299) using In-Fusion HD (Takara Bio) or NEBuilder HiFi (New England Biolabs), replacing the FLEX-mCherry construct originally in the vector. Descriptions of all the constructs tested are listed in Supplementary Table 1 . The constructs used for multiplexed EM labeling in the study are bolded in Supplementary Table 1 . All constructs were verified using Sanger sequencing and maintained in NEB Stable E. coli (New England Biolabs). Plasmids generated in this study (Table 1 ) were deposited to Addgene.
Aav expression vector
Manufactured by Takara Bio
The AAV expression vector is a laboratory tool designed to facilitate the production and study of adeno-associated viruses (AAVs). AAVs are small, non-pathogenic viruses that can be used as gene delivery vehicles. The AAV expression vector provides the necessary genetic components for the efficient generation and expression of recombinant AAV particles in a laboratory setting.
Automatically generated - may contain errors
2 protocols using aav expression vector
1
Synthesis, Cloning, and Verification of AAV Constructs
2
Synthesis, Cloning, and Verification of AAV Constructs
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DNA fragments were synthesized as IDT gBlocks Gene Fragments and/or oligonucleotides and amplified by PCR using Q5 Hot Start (New England Biolabs), and cloned into an AAV expression vector (Addgene plasmid # 20299) using In-Fusion HD (Takara Bio) or NEBuilder HiFi (New England Biolabs), replacing the FLEX-mCherry construct originally in the vector. Descriptions of all the constructs tested are listed in Supplementary Table 1 . The constructs used for multiplexed EM labeling in the study are bolded in Supplementary Table 1 . All constructs were verified using Sanger sequencing and maintained in NEB Stable E. coli (New England Biolabs). Plasmids generated in this study (Table 1 ) were deposited to Addgene.
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