C57BL/6J, TLR2−/−, and MyD88−/− mice were purchased from Jackson Laboratory and bred in-house and maintained at the University of Vermont animals care facility under protocols approved by Institutional Animal Care and Use Committee. Endotoxin free LPS (Escherichia coli serotype O), Pam2Csk4, Zymosan (Zy), Zymosan Depleted (ZD), Curdlan, and Whole Glucan Particles (WGP) were purchased from Invivogen. 2-deoxy-glucose (2DG) was purchased from Sigma. Antibodies for flow cytometry: 7-Aminoactinomycin D (7-AAD), anti-CD11c (clone N418), anti-CD86 (clone GL-1), and anti-CD40 (clone 3/23) antibodies were purchased from BD Biosciences and Biolegend. For Western blot analysis, all antibodies were from Cell Signaling [phosph-Akt T308 (clone D25E6), pan-Akt (clone C67E7), cleaved caspase-1 (clone E2G2I), and cleaved IL-1β (clone E7V2A) except for β-actin (clone 643802) which was purchased from Biolegend. PI3K (20 uM), Syk (10 uM) and TBK1 (5 uM) pharmacological inhibtors (LY294002, PRT062607, and BX-795 respectively) were purchased from Selleckchem.
β actin clone 643802
Manufactured by BioLegend
β-actin (clone 643802) is a monoclonal antibody that specifically recognizes the beta-actin isoform. It can be used for the detection and quantification of beta-actin in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd40 clone 3 23, by BD (3 mentions)
C57bl 6j, by Jackson ImmunoResearch (3 mentions)
Bx795, by Selleck Chemicals (2 mentions)
Myd88 mice, by Jackson ImmunoResearch (2 mentions)
Anti cd86 clone gl1, by BD (2 mentions)
Cleaved il 1β clone e7v2a, by BioLegend (2 mentions)
Prt062607, by Selleck Chemicals (2 mentions)
Ly294002, by Selleck Chemicals (2 mentions)
2 deoxyglucose 2 dg, by Merck Group (2 mentions)
Whole glucan particles, by InvivoGen (1 mentions)
Curdlan, by InvivoGen (1 mentions)
Pam2csk4, by InvivoGen (1 mentions)
Nigericin, by InvivoGen (1 mentions)
3 protocols using β actin clone 643802
1
Immune Response Signaling Pathways
2
Investigating Innate Immune Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J, TLR2−/−, and MyD88−/− mice were purchased from Jackson Laboratory (Bar Harbor, ME) and bred in‐house and maintained at the University of Vermont animals care facility under protocols approved by Institutional Animal Care and Use Committee. Endotoxin free LPS (Escherichia coli serotype O), Pam2Csk4, Zy, ZD, Curdlan, and WGP were purchased from Invivogen (San Diego, CA). 2‐deoxy‐glucose (2DG) was purchased from Sigma (St. Louis, MO). Antibodies for flow cytometry: 7‐Aminoactinomycin D (7‐AAD), anti‐CD11c (clone N418), anti‐CD86 (clone GL‐1), and anti‐CD40 (clone 3/23) antibodies were purchased from BD Biosciences (San Jose, CA) and Biolegend (San Diego, CA). For Western blot analysis, all antibodies were from Cell Signaling (Danvers, MA) (phosph‐Akt T308 [clone D25E6]), pan‐Akt (clone C67E7), cleaved caspase‐1 (clone E2G2I), and cleaved IL‐1β (clone E7V2A) except for β‐actin (clone 643802), which was purchased from Biolegend. PI3K (20 uM), Syk (10 uM), and TBK1 (5 uM) pharmacologic inhibitors (LY294002, PRT062607, and BX‐795 respectively) were purchased from Selleckchem (Houston, TX).
3
Endotoxin-induced immune responses in mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J were purchased from Jackson Laboratory and bred in-house and maintained at the University of Vermont animal care facility under protocols (IACUC # 18-027, approved on 19 August 2019) approved by the Institutional Animal Care and Use Committee. Endotoxin-free lipopolysaccharide (LPS, Escherichia coli serotype O), Pam2Csk4 (Pam), Zymosan (Zy), Zymosan depleted (ZD), and nigericin were purchased from InvivoGen (San Diego, CA). Glycogen phosphorylase inhibitor (PYGib) CP-91149 was purchased from Selleckchem (Houston, TX). Antibodies for flow cytometry: 7-Aminoactinomycin D (7-AAD), anti-CD11c (clone N418), anti-CD86 (clone GL-1), and anti-CD40 (clone 3/23) antibodies, were purchased from BD Biosciences (San Jose, CA) and BioLegend (San Diego, CA). For Western blot analysis, cleaved caspase-1 (clone E2G2I) and cleaved IL-1β (clone E7V2A) antibodies were from Cell Signaling, and β-actin (clone 643,802) was purchased from BioLegend.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!