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Red violet bile glucose medium

Manufactured by Thermo Fisher Scientific
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Sourced in United Kingdom

Red-violet bile glucose medium is a selective and differential culture medium used for the detection and enumeration of Enterobacteriaceae, particularly Escherichia coli and Salmonella species, in food, water, and other samples. The medium contains bile salts and crystal violet, which inhibit the growth of Gram-positive bacteria, and glucose, which is the fermentable carbohydrate. Colonies of Enterobacteriaceae that ferment glucose appear red-violet in color on this medium.

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Lab products found in correlation

Thiazolyl blue tetrazolium bromide, by Merck Group (3 mentions) Butylated hydroxytoluene, by Merck Group (3 mentions) Muller hinton agar, by Bio-Rad (3 mentions) Anhydrous sodium sulphate, by Merck Group (2 mentions) Linalool, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) K3po4, by Merck Group (1 mentions) Bis trimethylsilyl tri fluoroacetamide, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Pyridine, by Merck Group (1 mentions) α terpineol, by Merck Group (1 mentions) Mh agar plates, by Thermo Fisher Scientific (1 mentions) 1 8 cineole, by Merck Group (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions) α pinene, by Merck Group (1 mentions) Mh agar, by Thermo Fisher Scientific (1 mentions) Globulol, by Merck Group (1 mentions) Benzyl alcohol, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

3 protocols using red violet bile glucose medium

1

Antimicrobial activity assessment

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Anhydrous sodium sulphate, linalool ((±)-3,7-dimethyl-1,6-octadien-3-ol), butylatedhydroxytoluene (BHT), streptomycin, thiazolyl blue tetrazolium bromide (MTT), and potassium phosphate buffer (K3PO4) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Muller–Hinton agar was purchased from Bio-Rad, Marnes-la-Coquette, France. Agar plates of MH agar and red-violet bile glucose medium were obtained from Oxoid Ltd., Basingstoke, UK.
For extraction and derivatization, ethanol, pyridine, and bis-(trimethylsilyl) tri-fluoroacetamide (BSTFA) were purchased from Sigma-Aldrich (Steinheim, Germany).
Ben Akacha B., Garzoli S., Ben Saad R., Brini F., Mnif W., Kačániová M, & Ben Hsouna A. (2023). Biopreservative Effect of the Tunisian Halophyte Lobularia maritima Flavonoid Fraction, Used Alone and in Combination with Linalool in Stored Minced Beef Meat. Metabolites, 13(3), 371.
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2

Antimicrobial and Antioxidant Evaluation

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α‐Pinene, α‐terpineol, 1,8‐cineole, butylated hydroxytoluene (BHT), thiazolyl blue tetrazolium bromide (MTT), and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) were purchased from Sigma‐Aldrich Co. Muller–Hinton agar was purchased from Bio‐Rad. MH agar plates and red–violet bile glucose medium were obtained from Oxoid Ltd.
Ben Akacha B., Michalak M., Generalić Mekinić I., Kačániová M., Chaari M., Brini F., Ben Saad R., Mnif W., Garzoli S, & Ben Hsouna A. (2023). Mixture design of α‐pinene, α‐terpineol, and 1,8‐cineole: A multiobjective response followed by chemometric approaches to optimize the antibacterial effect against various bacteria and antioxidant activity. Food Science & Nutrition, 12(1), 574-589.
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3

Antimicrobial Compound Evaluation Protocol

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Anhydrous sodium sulphate, benzyl alcohol, linalool, terpien-4-ol, globulol, dimethyl sulfoxide (DMSO), butylated hydroxytoluene (BHT), streptomycin, thiazolyl blue tetrazolium bromide (MTT), potassium phosphate buffer (K3PO4) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Muller–Hinton agar was purchased from Bio-Rad(Steenvoorde, France). Agar plates of MH agar and red-violet bile glucose medium were attained from Oxoid Ltd., Basingstoke, UK.
Ben Akacha B., Švarc-Gajić J., Elhadef K., Ben Saad R., Brini F., Mnif W., Smaoui S, & Ben Hsouna A. (2022). The Essential Oil of Tunisian Halophyte Lobularia maritima: A Natural Food Preservative Agent of Ground Beef Meat. Life, 12(10), 1571.
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