Tissue culture reagents (RPMI-1640, fetal bovine serum, penicillin, streptomycin, and L-glutamine) were from Gibco BRL. The tissue culture incubator (water jacket CO2 incubator, Forma Series II, Model 3100 Series) was from Thermo Fisher. The tissue culture workstation (Logic+ A2 hood) was from Labconco (Kansas City, MO, USA). Tissue culture plastic (flasks, plates, cell scraper) was from CytoOne (USA Scientific, Ocala, FL, USA). Cell counting reagent (trypsin blue dye), slides (dual chamber), counter (TC20 Automatic Cell Counter), and microscope (Zoe Florescent Cell Imager) were from Bio-Rad (Hercules, CA, USA). The microplate spectrophotometer (Epoch) was from BioTek Instruments (Winooski, VT, USA).
Logic a2 hood
Manufactured by Labconco
Sourced in United States
The Logic + A2 hood is a lab equipment product manufactured by Labconco. It is designed to provide primary containment and personal protection for the user when working with hazardous materials. The hood features a transparent front sash and side panels to allow visibility and access during use.
Automatically generated - may contain errors
Lab products found in correlation
Tc20 automatic cell counter, by Bio-Rad (4 mentions)
Zoe florescent cell imager, by Bio-Rad (4 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Trypsin blue dye, by Bio-Rad (3 mentions)
Epoch microplate spectrophotometer, by Agilent Technologies (3 mentions)
Mtt based in vitro toxicology assay kit, by Merck Group (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Gossypol, by Merck Group (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
4 protocols using logic a2 hood
1
Tissue Culture Reagents and Equipment
2
MTT-based Cytotoxicity Assay Protocol
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Cell cytotoxicity reagent (MTT based-In Vitro Toxicology Assay Kit), gossypol, LPS and other chemicals (chloroform, DMSO, ethanol, hexane, sodium bicarbonate, sulfuric acid) were from Sigma (St. Louis, MO). Tissue culture reagents (DMEM, MEM, fetal bovine serum, penicillin, streptomycin, L-glutamine) were from Gibco BRL (Thermo Fisher). Tissue culture incubator was water jacket CO2 incubator, Forma Series II, Model 3100 Series (Thermo Fisher). Tissue culture workstation was Logic + A2 hood (Labconco, Kansas City, MO). Tissue culture plastic ware (flasks, plates, cell scraper) was from CytoOne (USA Scientific, Ocala, FL). Cell counting reagent (trypsin blue dye), slides (dual chamber), counter (TC20 Automatic Cell Counter) and microscope (Zoe Florescent Cell Imager) were from Bio-Rad (Hercules, CA). Molecular sieves were from USA Standard Testing Sieves (Milwaukee, WI). Microplate spectrophotometer (Epoch) was from BioTek Instruments (Winooski, VT).
3
In Vitro Cytotoxicity Assay Protocol
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Cell cytotoxicity reagent (MTT based-In Vitro Toxicology Assay Kit) and DMSO were from Sigma. Tissue culture reagents were from Gibco BRL (Thermo Fisher). Tissue culture incubator was water jacket CO2 incubator (Thermo Fisher). Tissue culture workstation was Logic + A2 hood (Labconco, Kansas City, MO). Tissue culture plastic ware was from CytoOne (USA Scientific, Ocala, FL). Cell counting reagent (trypsin blue dye), slides (dual chamber), counter (TC20 Automatic Cell Counter) and microscope (Zoe Florescent Cell Imager) were from Bio-Rad (Hercules, CA). Microplate spectrophotometer (Epoch) was from BioTek Instruments (Winooski, VT).
4
Gossypol Cytotoxicity Assay in Cells
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The chemicals, reagents and equipment were described essentially as previously56 (link). Gossypol (molar mass: 518.56 g/mol) was purified from cottonseed by HPLC and purchased from Sigma (St. Louis, MO). Gossypol stock was prepared in 100% DMSO at 10 mg/mL (approximately 19.2 mM). Cell cytotoxicity reagent (MTT based-In Vitro Toxicology Assay Kit) and DMSO were from Sigma. Tissue culture reagents (RPMI-1640, fetal bovine serum, penicillin, streptomycin, L-glutamine) were from Gibco BRL (Thermo Fisher). Tissue culture incubator was water jacket CO2 incubator, Forma Series II, Model 3100 Series (Thermo Fisher). Tissue culture workstation was Logic + A2 hood (Labconco, Kansas City, MO). Tissue culture plastic ware (flasks, plates, cell scraper) was from CytoOne (USA Scientific, Ocala, FL). Cell counting reagent (trypsin blue dye), slides (dual chamber), counter (TC20 Automatic Cell Counter) and microscope (Zoe Florescent Cell Imager) were from Bio-Rad (Hercules, CA). Microplate spectrophotometer (Epoch) was from BioTek Instruments (Winooski, VT).
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