Monocytes adhering to EA.hy926 cells were evaluated by using THP‐1 cells as previously described.28 Briefly, EA.hy926 cells were treated with TDD (0, 5, 10 and 20 μmol/L) for 4 hours before the addition of LPS (1 μg/mL) for 12 hours in 96‐well plates. Calcein AM‐labelled THP‐1 cells were then added to EA.hy926 cells for 1 hour and non‐adherent THP‐1 cells were removed by washing with PBS. The fluorescence of bound monocytes was measured using a multi‐detection microplate reader (Variskan Flash; Thermo Scientific, Waltham, MA, USA) at 490 and 515 nm.
Variskan flash
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Variskan Flash is a compact and versatile laboratory instrument designed for rapid, high-throughput fluorescence measurements. The core function of the Variskan Flash is to provide efficient and accurate fluorescence detection capabilities for a wide range of applications.
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2 protocols using variskan flash
1
Monocyte Adhesion Dynamics in EA.hy926 Cells
2
Comprehensive Analytical Techniques for Biomolecular Characterization
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Gel electrophoresis analysis was carried out on a Bio-Rad Electrophoresis System and imaged on Bio-Rad ChemDoc XRS (Bio-Rad, USA). The fluorescence spectra and CCK-8 assay were recorded using a Thermo Scientific Variskan Flash (Thermo, USA). RT-PCR was performed on an ABI 7500 Real-Time PCR machine (ABI, Applied Biosystems, USA). The confocal fluorescence imaging was performed on a Zeiss LSM780 laser scanning confocal microscope (Zeiss, Germany).
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