Myelin basic protein (M1891–5MG), Menadione (M5625) and AMP-PNP (A2647) were purchased from Sigma. Selenomethionine containing medium was purchased from Molecular Dimensions (MD12–500). Tris(2-carboxyethyl)phosphine hydrochloride (TCEP; 20490) and 4-acetamido-4’-maleimidylstilbene-2,2’- disulfonic acid (AMS; A485) were purchased from Thermo Fisher. Biotin-17-ATP was purchased from Enzo life sciences (ENZ-42817). High sensitivity streptavidin HRP (21130) and high capacity streptavidin agarose resin (20357) were purchased from Pierce. Rabbit anti-GroEl antibodies were purchased from Enzo life sciences (ADI-SPS-875-D). Rabbit anti-Vma6 antibodies were a generous gift from Dr. Vincent Starai (University of Georgia, Athens). Mouse anti-porin antibodies were from Invitrogen (459500), and rabbit anti-histone H3 antibodies were from Millipore (06–755). Rabbit anti-Mia40 antibodies were a generous gift from Dr. Carla Koehler (UCLA). Rabbit anti AMP-Threonine antibodies were as described previously (Hao et al., 2011 (link)). Mouse ant-GSH and anti-GAPDH antibodies were from Thermo.
Tris 2 carboxyethyl phosphine hydrochloride tcep 20490
Manufactured by Thermo Fisher Scientific
Tris(2-carboxyethyl)phosphine hydrochloride (TCEP; 20490) is a reducing agent used for the reduction of disulfide bonds in proteins and peptides. It is a water-soluble, odorless, and non-toxic compound.
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2 protocols using tris 2 carboxyethyl phosphine hydrochloride tcep 20490
1
Protein Purification and Analysis
2
Comprehensive Biochemical Assay Protocol
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ATP (A2383), CTP, GTP, ɑ-ketoglutaric acid (75890) L-glutamic acid (G1626), NAD+ (10127965001) Etheno-NAD+ (N2630) NADH (10107735001) NADP+ (93205), protease inhibitor cocktail (PIC; 11873580001), and nicotinamide (N3376) were from Sigma. Six-biotin-17-NAD+ (4670-500-01) was from Trevigen (for capture of Lart1 substrate in yeast lysate) and Amsbio (AMS.80610, for assays with Lart1 orthologs). [32P]-NAD (NEG023X250UC) was from Perkin Elmer. Tris (2-carboxyethyl) phosphine hydrochloride (TCEP; 20490), PfuTurbo DNA polymerase (50-125-946), and high-capacity streptavidin agarose (20357) were purchased from Thermo Fisher Scientific. High-sensitivity streptavidin HRP (21130) was from Pierce. The ethenoadenosine antibody 1G4 (sc-52666) was from Santa Cruz Biotechnology. Q5 DNA polymerase, 2X Gibson assembly mix, and all restriction enzymes used for cloning were from New England Biolabs.
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