Primary antibodies used in Western blot were rabbit polyclonal antiadvillin (1 μg/mL), mouse monoclonal anti–β-actin (1:10000; Sigma), mouse anti–N-cadherin (1:1,000; Millipore), mouse monoclonal antimyosin IIa (1:500, ab55456; Abcam), and rabbit IgG isotype control (1 μg/mL), and rabbit antialbumin (1:5,000, both GeneTex). Details are described in SI Appendix .
Rabbit antialbumin
Manufactured by GeneTex
Rabbit antialbumin is a laboratory reagent used for the detection and quantification of albumin, a common protein found in various biological samples. It is a polyclonal antibody produced by immunizing rabbits with albumin. The primary function of this product is to provide a specific binding partner for albumin, allowing for its identification and measurement through techniques such as immunoassays.
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2 protocols using rabbit antialbumin
1
Western Blot Primary Antibodies
2
Immunofluorescent Staining of Cell Markers
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After PAS staining, the cells were washed and blocked with 5% goat serum (16210-064, Thermo Fisher Scientific) in PBST (PBS- supplemented with 0.1% Tween 20) for 30 min. The cells were incubated with 1/800 dilution of mouse anti-cadherin (610181, BD Biosciences) and rabbit anti-albumin (102419, GeneTex) antibodies in PBST for 1 h. After washing with PBST, the cells were incubated with goat anti-mouse IgG Alexa Flor555 (A21422, Thermo Fisher Scientific) and goat anti-rabbit IgG Alexa Flor 488 (111–545-144, Jackson ImmunoResearch) for 60 min. Nuclei were stained with a 1/10000 dilution of Hoechst 33342(H3570, Thermo Fisher Scientific) in PBS, after incubation with secondary antibody. Finally, the cells were washed with PBS and photographed with a fluorescent microscopy (IX71, Olympus).
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