Anti phosphotyrosine antibody py100

Manufactured by Cell Signaling Technology

Anti-phosphotyrosine antibody (PY100) is a primary antibody used in various research applications. Its core function is to detect and bind to proteins containing phosphorylated tyrosine residues, which are important in cellular signaling pathways.

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Lab products found in correlation

Human thrombin, by Merck Group (1 mentions) Anti syk antibody, by Cell Signaling Technology (1 mentions) Anti phospho src, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-phosphotyrosine antibodies PY100

2 protocols using anti phosphotyrosine antibody py100

Glycated-BSA-induced AGE signaling

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Glycated-BSA, used as the AGE in this study, was purchased from Bio Vision. Human thrombin was obtained from Sigma-Aldrich. Prostaglandin E1 (PGE1), anti-c-Src and anti-phospho-Src antibodies were purchased from Santa Cruz Biotechnology. BAPTA-AM and PP2 were obtained from Dojin and Abcam, respectively. Anti-Syk antibody and anti-phosphotyrosine antibody (PY100) were purchased from Cell Signaling Technology.

Furukawa K., Fuse I., Iwakura Y., Sotoyama H., Hanyu O., Nawa H., Sone H, & Takei N. (2017). Advanced glycation end products induce brain-derived neurotrophic factor release from human platelets through the Src-family kinase activation. Cardiovascular Diabetology, 16, 20.

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Phosphotyrosine Peptide Enrichment Protocol

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The phosphotyrosine peptide enrichment was performed according to the manufacturer’s protocol (Cell Signaling Technology). Briefly, lyophilized peptides were reconstituted in immunoaffinity purification buffer containing 50 mM MOPS pH 7.2, 10 mM sodium phosphate, 50 mM NaCl. Antiphosphotyrosine antibody (pY100, Cell Signaling Technology) was added to the peptide solution and rotated at 4°C for 30 min. Postincubation, antibody and peptide complexes were washed twice with immunoaffinity purification buffer and water. The phosphopeptides were eluted using 0.1% TFA. The eluted phosphopeptides were desalted using C₁₈ STAGE tips, vacuum dried, and kept at −80°C before LC–MS analysis.

Blair B.G., Wu X., Zahari M.S., Mohseni M., Cidado J., Wong H.Y., Beaver J.A., Cochran R.L., Zabransky D.J., Croessmann S., Chu D., Toro P.V., Cravero K., Pandey A, & Park B.H. (2014). A phosphoproteomic screen demonstrates differential dependence on HER3 for MAP kinase pathway activation by distinct PIK3CA mutations. Proteomics, 15(0), 318-326.

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