Primary mouse monoclonal immunoglobulin G2a (IgG2a) anti-gpK8.1 (clone 4A4; Santa Cruz Biotechnology, Dallas, TX) [22 (link)], which detects the gpK8.1 ectodomain (ED), was used for fluorescence-activated cell sorting (FACS), immunoblot, and enzyme-linked immunosorbent assay (ELISA). Primary polyclonal rabbit anti-Newcastle disease virus (NDV) detecting nucleoprotein (NP) was a gift of Dr. T. Morrison, University of Massachusetts Medical School, Worcester. Primary polyclonal goat anti-human IgG Fc or rabbit anti-2A peptide were purchased from ThermoFisher and MilliporeSigma, respectively. Primary monoclonal anti-bodies (mAbs) anti-gpK8.1 (clone 41E7) and anti-gH/gL (clones 54A1, 57C12) were generated and characterized as outlined below and used in immunoblot and ELISA. HRP-conjugated secondary antibodies goat anti-mouse IgG and goat anti-rabbit IgG (MilliporeSigma) were used for immunoblot and ELISA. Goat Fab-2 anti-mouse IgG (H+L) cross-adsorbed secondary antibody, conjugated to Alexa Fluor 488 (AF488) (ThermoFisher) was used for FACS.
Goat anti human igg fc
Manufactured by Thermo Fisher Scientific
Sourced in Germany
Goat anti-human IgG Fc is a laboratory reagent used to detect and measure the presence of human immunoglobulin G (IgG) in biological samples. It is a polyclonal antibody raised in goats against the Fc region of human IgG. This product can be used in various immunoassay techniques to quantify or identify human IgG levels.
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Lab products found in correlation
Alexa fluor 488 af488, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igg, by Merck Group (1 mentions)
Goat anti rabbit igg, by Merck Group (1 mentions)
Mab1859, by R&D Systems (1 mentions)
Nf κb p65 c 20, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase hrp conjugated antibody, by Thermo Fisher Scientific (1 mentions)
2 protocols using goat anti human igg fc
1
Primary Mouse Monoclonal IgG2a Anti-gpK8.1
2
Dectin-1 Signaling Pathway Analysis
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All materials were purchased from Sigma or Merck, unless otherwise stated. Primary antibodies were mouse anti-human Dectin-1 clone GE-2 (kindly provided by Gordon Brown, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom), mouse anti-human Dectin-1 clone MAB1859 (R&D Systems), monoclonal antibody (MAb) Be9.2 (as the isotype control; kindly provided by W. Reutter, Charite-Universitätsmedizin Berlin, Germany), NF-κB p65 (C-20; Santa Cruz Biotechnology), polymerase II (N-20; Santa Cruz Biotechnology), goat anti-human IgG-Fc, and horseradish peroxidase (HRP)-conjugated antibody (Thermo Scientific). Secondary antibodies were phycoerythrin (PE)-labeled goat anti-mouse antibody (antibodies-online GmbH) and HRP-coupled goat anti-mouse antibody (Dianova).
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