Tiletamine zolazepam

Animal welfare and experimental procedures were carried out in accordance with the Guide for the Care and Use of Laboratory Animals (Ministry of Science and Technology of China, 2006), and were approved by the animal ethics committee of Nanjing Medical University. Healthy specific pathogen-free (SPF) purpose-bred domestic C57BL/6 mice (6-weeks-old, 15–23 g body weight) were obtained from the Model Animal Research Center of Nanjing University, Jiangsu, China. Animals had ad libitum access to rodent diet and tap water. The mice were kept in cages with a 12:12-h light–dark cycle, a temperature of 21 °C ±2 °C, and a humidity of 55 % ±5 %.
Mice assigned to the unilateral ureteral obstruction (UUO) model were anesthetized by tiletamine/zolazepam (VIRBAC Laboratories, Carros, France) and placed on a heating pad to maintain their temperature at 37 °C. Left ureters were ligated with silk (4/0). Amoxicillin was given to the animals after surgery for three days. Sham animals underwent the same procedure, except that the ureter was not ligated. The animals were randomly divided into four groups: sham group (n = 10), UUO group (n = 10), UUO + MVs group (n = 10), and UUO + EPO-MVs group (n = 10). The mice were injected in the tail vein with MVs or EPO-MVs (30 μg/mouse) one day after surgery The four groups were killed at 7 days and 14 days after UUO.

The genital CTVT mass of a 4-year-old mongrel male dog was diagnosed by cytological and polymerase chain reaction (PCR) examination (Supplementary Figure 1), as reported (31 (link)). This dog was intravascularly injected with tiletamine-zolazepam (Zoletil®, Virbac, France) at a dosage of 4 mg/kg body weight (BW). The tumor tissue was aseptically biopsied before commencing chemotherapeutic treatment on the dog, as weekly intravenous injection of vincristine sulfate at 0.025 mg/kg BW. Sampling procedures were approved by the Chulalongkorn University Animal Care and Use Committee (No. 133100077). Biopsied tissue was immediately immersed in Hank's buffered salt solution (HBSS; Gibco Lab, USA) containing 100 IU/ml penicillin and 100 μg/ml streptomycin (Gibco Laboratories, USA). The dog's hematology and serum chemistry profile were in the normal range prior to receiving chemotherapeutic treatment.