Insulin elisa kit

Food intake and body weight were measured once a week. Briefly, for the glucose tolerance test (GTT), mice which were fasted overnight were injected (i.p.) with D-glucose (1 g/kg body weight; Sigma-Aldrich) after 12 h fasting. For the insulin tolerance test (ITT), mice were injected (i.p.) with insulin (0.75 units/kg body weight; Novolin R, Novo Nordisk, Copenhagen, Denmark) after 6 h fasting. Glucose concentrations were measured in tail venous blood using an automated glucometer (Roche Diagnostics) up to 90 min or 120 min after injection.
Fasting insulin was measured by the insulin ELISA kit (Cusabio, Wuhan, China). Liver concentrations of triglyceride (TG) was measured by the triglyceride assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Serum concentrations of total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), apolipoprotein E (ApoE), FFA and hsCRP were measured by enzymatic method using an automatic biochemical analyzer (Beckman AU5800, USA).

For the evaluation of glucose tolerance, all the rats were given glucose orally (2.0 g/kg BW) after overnight fasting prior that the animals were euthanized. Blood samples were collected from the tail vein at 0, 15, 30, 60, 90 and 120 min, and glucose was measured using a glucose metre (Roche, Germany). The serum was centrifuged at 4℃ (4000 rpm, 900 × g) for 15 min and stored at −80℃ for assay. Serum insulin level was determined by commercially available insulin ELISA kit (CUSABIO, Wuhan). The zero‐time value was taken as the fasting blood glucose value and fasting insulin value. The homeostatic model assessment of insulin resistance (HOMA‐IR) index was calculated according to the following formulas:
HOMA‐IR = (FBG × FINS)/22.5,
where FBG is the fasting blood glucose level and FINS is the fasting insulin level.
The insulin sensitivity index was calculated by the ratio of area under the glucose curve to area under the insulin curve (AUCg/AUCi).

FINS were measured by using an enzyme-linked immunosorbent assay insulin ELISA kit (CSB-E05070r, CUSABIO BIOTECH Co., Ltd, Wuhan, China). Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) was applied to assess insulin resistance by the following formula: HOMA score = fasting serum insulin (μU/mL) × fasting glucose (mmol/L)/22.5 [13 (link), 14 (link)]. After logarithmic transformation the statistical analysis was performed.