Anti mouse alexa fluor 488 labeled

Manufactured by Thermo Fisher Scientific

The Anti-mouse Alexa Fluor 488-labeled is a fluorescently-labeled secondary antibody used in various immunodetection techniques. Alexa Fluor 488 is a green-fluorescent dye that can be excited at 488 nm and emits light at around 520 nm. This product is designed to detect and visualize primary antibodies raised against mouse antigens.

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Lab products found in correlation

Hoechst dye, by Santa Cruz Biotechnology (2 mentions) Ap308p, by Merck Group (1 mentions) Ap307p, by Merck Group (1 mentions) 10 ncp, by Merck Group (1 mentions) Lysotracker green dnd 26, by Thermo Fisher Scientific (1 mentions) Doxorubicin, by Selleck Chemicals (1 mentions) Pmxs puro gfp p62, by Addgene (1 mentions) Pegfp n1 tfeb, by Addgene (1 mentions)

Spelling variants of this product

Alexa Fluor 488 (6806 citations) Alexa 488 (1863 citations) Anti-mouse Alexa Fluor 488 (195 citations) Anti-mouse Alexa-488 (181 citations) Alexa Fluors (21 citations) Alexa Fluor 488 anti (6 citations) Anti-mouse Alexa Fluor (4 citations) Fluor 488 labeled anti-mouse (2 citations) Alexa Fluor 488 labeled anti-rabbit or anti-mouse secondary antibodies (2 citations) Alexa Fluor 488‐labeled donkey anti‐mouse immunoglobulin G (IgG) (2 citations)

2 protocols using anti mouse alexa fluor 488 labeled

Detailed Reagents and Constructs

Cited 4 times

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PDS was from Cayman Chemical (#18013). 10-NCP (10-(4′-(N-diethylamino)butyl)−2-chlorophenoxazine) was from EMD Millipore ((#925681–41). Hoechst dye was from Santa Cruz Biotechnology (#sc-394039). N-TASQ was synthesized as described (Laguerre et al., 2015 (link); Laguerre et al., 2016 (link); Yang et al., 2017 (link)). Antibodies against LC3 were from MBL (#PD014). Antibodies against ATG7 (D12B11; #8558), β-actin (8H10D10; #3700), and the Anti-FLAG DYKDDDDK M2 tag (D6W5B; #2368) were from Cell Signaling. Mouse antibodies against MAP2c (A-4, #sc-74421) were from Santa Cruz Biotechnology. Antibodies against Htt (mEM48), rabbit IgG(H+L) conjugated with horseradish peroxidase (HRP) (#AP307P), and mouse IgG(H+L) conjugated with HRP (#AP308P) were from EMD Millipore. Anti-mouse Alexa Fluor 488-labeled (#A11001) and anti-rabbit Alexa Fluor 546-labeled (#A11010) secondary antibodies were from Life Technologies. A single-chain BG4 antibody that recognizes G4 structures (Biffi et al., 2013 (link)) was purified in the lab of Dr. Nayun Kim. pGW1-Dendra2-LC3 was described (Tsvetkov et al., 2013b (link)). pGW1-mito-Keima was cloned from the mt-mKeima/pIND(SP1) construct that was received from Dr. Atsushi Miyawaki (RIKEN Brain Science Institute, Japan). pCAG-TagBFP, pCAG-EGFP-PIF1, pCAG-EGFP-mPIF1 (E307Q George et al., 2009 (link)), and pEF1A-mApple-ATG7 were cloned by VectorBuilder.

Moruno-Manchon J.F., Lejault P., Wang Y., McCauley B., Honarpisheh P., Morales Scheihing D.A., Singh S., Dang W., Kim N., Urayama A., Zhu L., Monchaud D., McCullough L.D, & Tsvetkov A.S. (2020). Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons. eLife, 9, e52283.

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Doxorubicin and Autophagy Modulation

Cited 1 time

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Doxorubicin was from Selleckchem (Houston, TX). HPβCD was from Cayman Chemical Company (Ann Arbor, MI). 10-(4′-(N-diethylamino)butyl)-2-chloro-phenoxazine hydrochloride (10-NCP) was from Calbiochem (San Diego, CA). NH4Cl was from Biomatik (Wilmington, DE). Lysotracker Green DND-26 was from Life Technologies (Carlsbad, CA). Antibodies against actin (#3700, 1:1.000) and p62 (#5114, 1:1.000) were from Cell Signaling (Danvers, MA). Antibody against tubulin (#ab7751, 1:1.000) was from Abcam (Cambridge, MA). Antibody against MAP2c (#sc-74421, 1:100) was from Santa Cruz Biotechnologies (Dallas, TX), and antibody against LC3 (#PD014, 1:1.000) was from MBL (Woburn, MA). Anti-mouse Alexa Fluor 488-labeled (#A11001, 1:200) was from Life Technologies. Hoechst dye was from Santa Cruz Biotechnology. pEGFP-N1-TFEB was from Addgene (#38119, deposited by Dr. Shawn Ferguson, Yale School of Medicine). pGW1-beclin1-GFP was described [37 (link)]. pGW1-GFP-p62 was cloned from pMXs-puro GFP-p62, which was from Addgene (#38277, deposited by Dr. Noboru Mizushima, the University of Tokyo).

Moruno Manchon J.F., Uzor N.E., Kesler S.R., Wefel J.S., Townley D.M., Nagaraja A.S., Pradeep S., Mangala L.S., Sood A.K, & Tsvetkov A.S. (2016). TFEB ameliorates the impairment of the autophagy-lysosome pathway in neurons induced by doxorubicin. Aging (Albany NY), 8(12), 3507-3519.

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