Cmra cell tracker dye

Manufactured by Thermo Fisher Scientific

Sourced in United States

The CMRA cell tracker dye is a fluorescent dye that can be used to label and track live cells. It binds to cellular components, allowing researchers to visualize and monitor cell populations over time.

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Lab products found in correlation

Triton x 100, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Avantor (1 mentions) Pbs tween 20, by Thermo Fisher Scientific (1 mentions) Lsm 710, by Zeiss (1 mentions)

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Cell Tracker dyes (7 citations)

2 protocols using cmra cell tracker dye

Live Cell Tracking and Immunostaining

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CMRA cell tracker dye (ThermoFisher Scientific, MA, USA) was diluted to a concentration of 25 μM in cell media and added to the culture flask to enable live tracking of cells. The cells were incubated with the dye for 30 minutes, followed by replacing the media containing the CMRA stain with a culture medium.
For immunostaining, the cells were fixed with 4% paraformaldehyde (ThermoFisher Scientific, MA, USA) and permeabilized with 0.1% Triton X-100 (J63521, Alfa Aesar, MA, USA) for 15 minutes. The permeabilized cells were then blocked with 4% Bovine Serum Albumin (BSA) (VWR, PA, USA) for 30 minutes, and each step was followed by washing the cells with PBS Tween 20 (ThermoFisher Scientific, MA, USA).

Joshi I.M., Mansouri M., Ahmed A., Simon R.A., Bambizi P.E., Desa D.E., Elias T.M., Brown E.B, & Abhyankar V.V. (2023). Microengineering 3D Collagen Matrices with Tumor-Mimetic Gradients in Fiber Alignment. bioRxiv.

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Quantifying Cell-Cell Interactions with Imaging

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Cell-cell mixing experiments and staining of cells was carried out as described previously.⁴ (link)^,¹⁰ (link) In brief, one cell population was labeled with CMRA cell tracker dye (ThermoFisher Scientific) as previously described,⁴ (link) before mixing with unlabelled cells at 1:50 ratios. After 48 hours, cells were fixed in 4% PFA for 15 min, before 15 min permeabilization in 0.25% Triton X-100/PBS and blocking in 3% BSA/PBS for 1 hour. Cells were then incubated in anti-E-cadherin antibody (Key resources table), diluted in blocking buffer, overnight at 4°C. The following day, cells were washed three times with PBS and incubated in secondary antibodies (1:200, Life Technologies) and phalloidin (1:200) for 1 h at room temperature. Cells were then mounted in Mowiol and imaged on a Zeiss confocal LSM 710.

Hill W., Zaragkoulias A., Salvador-Barbero B., Parfitt G.J., Alatsatianos M., Padilha A., Porazinski S., Woolley T.E., Morton J.P., Sansom O.J, & Hogan C. (2021). EPHA2-dependent outcompetition of KRASG12D mutant cells by wild-type neighbors in the adult pancreas. Current Biology, 31(12), 2550-2560.e5.

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