Adipogenesis differentiation kit
The Adipogenesis Differentiation Kit is a laboratory tool designed to facilitate the in vitro differentiation of adipocytes from precursor cells. The kit provides a defined media formulation and optimized protocols to support the stepwise process of adipogenesis.
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10 protocols using adipogenesis differentiation kit
Multilineage Differentiation of Mesenchymal Stem Cells
Multilineage Differentiation of muBM-MSCs
Adipogenesis Differentiation Assay
Adipogenic Differentiation Assay
Staining was made of 99% isopropanol and Oil Red O (Sigma-Aldrich). The resulting solution was diluted in distilled water (3 : 2). Isopropanol was removed and the staining was added after 10 minutes of incubation on the cells for 5 minutes to verify positive effect of differentiation.
Adipogenesis Differentiation and Staining
Staining was achieved with 99% isopropanol plus Oil Red O (Sigma-Aldrich, Saint Louis, MO, USA). The resulting solution was diluted in distilled water (3:2). Isopropanol was removed and the stain added after 10 min of incubation of the cells for 5 min to verify the positive effect of differentiation.
Multilineage Differentiation of Stem Cells
Multilineage Differentiation of Mouse MSCs
To evaluate their multipotent differentiation potential, MSCs were incubated at passage 5 in a 12‐well plate at a density of 5 × 103 cells/cm2 with osteogenesis differentiation medium (Gibco) or at a density of 1 × 104 cells/cm2 with an adipogenesis differentiation kit (Gibco) for 3 weeks. According to the manufacturer's instructions, other MSCs were seeded at a higher density of 1 × 107 cells per milliliter to formulate a micromass and cultured with a STEMPRO chondrogenesis differentiation kit (Gibco) with medium changes every 3 days for 2 weeks. Osteoblasts were stained using Alizarin Red S (Sigma), adipocytes were stained with Oil Red O (Sigma), and chondrogenesis pellets were stained with Toluidine Blue (Sigma).
Adipogenesis Differentiation of MSCs
Multilineage Differentiation of UC-MSCs
Multilineage Differentiation of Bcl-2-ADSCs
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