A PP nonwoven fabric was ordered from Langfang Huijing Paper Plastics Manufacture Inc. This fabric was washed with excess acetone and then dried at room temperature. ITX was obtained from TH-UNIS Insight Co., Ltd. Citric acid monohydrate and trisodium citrate dihydrate was purchased from Sinopharm Chemical Reagent Co., Ltd. Cellulase was obtained from Trichoderma reesei (EC 3.2.1.4), β-glucosidase from almonds (EC 3.2.1.21), PEGDA with a molecular weight of 575 was provided by Sigma-Aldrich Chemical Co. FUN® 1 and Calcofluor® was obtained from Life Technologies Co. Filter paper (used as a cellulose model) was ordered from Hangzhou Special Paper Co., Ltd. Other chemicals were purchased from Alfa Aesar Chemical Co.
Calcofluor
Manufactured by Thermo Fisher Scientific
Calcofluor is a fluorescent dye used in microscopy for the staining and visualization of cellulose and chitin in biological samples. It binds to these polysaccharides and emits a blue-white fluorescence under ultraviolet or blue light illumination, allowing the identification and observation of cell walls and fungal structures.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by Thermo Fisher Scientific (2 mentions)
Tcs sp5 2 aobs confocal laser scanning microscope, by Leica (2 mentions)
Shandon cryomatrix, by Thermo Fisher Scientific (2 mentions)
Dfc310 fx digital camera, by Leica (2 mentions)
Syto9, by Thermo Fisher Scientific (2 mentions)
Cellulase, by Merck Group (1 mentions)
Citric acid monohydrate, by Sinopharm (1 mentions)
Fun 1, by Thermo Fisher Scientific (1 mentions)
β glucosidase, by Merck Group (1 mentions)
Pegda, by Merck Group (1 mentions)
Trisodium citrate dihydrate, by Sinopharm (1 mentions)
Cm1850, by Leica (1 mentions)
M205 fa, by Leica (1 mentions)
3 protocols using calcofluor
1
Enzymatic Hydrolysis of Cellulose Fabric
2
Nodule Imaging and Microscopy Protocol
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Images of whole-mount nodulated roots were captured using a Leica stereo fluorescence microscope M205FA equipped with a Leica DFC310FX digital camera (Leica Microsystems). Detached nodules were embedded in Shandon cryomatrix (Thermo scientific) and were sliced into 30-µm thick sections with a rotary cryomicrotome CM1850 (Leica Microsystems). For confocal microscopy, sample preparation was done according to Haynes and associates (2004) . Sections were stained with Calcofluor (Life Technologies), propidium iodide (Life Technologies), and Syto9 (Life Technologies). Images were acquired with a Leica TCS SP5 II AOBS confocal laser scanning microscope (Leica Microsystems). For confocal and scanning electron microscopy, sample preparation was done according to (Kundu and DasGupta 2018) . All digital micrographs were processed using Adobe Photoshop CS5.
3
Whole-Mount Imaging of Nodulated Roots
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Images of whole-mount nodulated roots were captured using a Leica M205FA stereofluorescence microscope equipped with a Leica DFC310FX digital camera (Leica Microsystems). Detached nodules were embedded in Shandon cryomatrix (Thermo Scientific) and sliced into 30-µm-thick sections with a CM1850 rotary cryomicrotome (Leica Microsystems). For confocal microscopy, sample preparation was done according to Haynes and associates (2004) and Sinharoy and DasGupta (2009) . Sections were stained with Calcofluor (Life Technologies), propidium iodide (Life Technologies) and Syto9 (Life Technologies). Images were acquired with a Leica TCS SP5 II AOBS confocal laser-scanning microscope (Leica Microsystems). All digital micrographs were processed using Adobe Photoshop CS5.
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