Expression vectors encoding the Yap1 shRNA and scrambled control shRNA were ordered from Open Biosystems, Inc. Lentivirus was produced by a four-plasmid (for 3rd-generation lentiviral vectors) transfection system as described by Tiscornia et al. (Tiscornia et al., 2006 (link)). Lentivirus was collected in keratinocyte serum-free medium and directly used to transduce primary keratinocytes overnight. Animals and other detailed methods are shown in the Supplementary Materials and Methods . All animal experiments were approved by Institutional Animal Care and Use Committee the Animal Care Committee at the University of Louisville (IACUC #12005)
Expression vectors encoding the
Manufactured by Thermo Fisher Scientific
Expression vectors encoding the are laboratory tools used to produce specific proteins in cells. They contain the necessary genetic elements, such as a promoter and coding sequence, to drive the expression of a target gene of interest. These vectors provide a standardized platform for recombinant protein production in various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using expression vectors encoding the
1
Lentiviral Transduction of Primary Keratinocytes
2
Lentiviral Transduction of Primary Keratinocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Expression vectors encoding the Yap1 shRNA and scrambled control shRNA were ordered from Open Biosystems, Inc. Lentivirus was produced by a four-plasmid (for 3rd-generation lentiviral vectors) transfection system as described by Tiscornia et al. (Tiscornia et al., 2006 (link)). Lentivirus was collected in keratinocyte serum-free medium and directly used to transduce primary keratinocytes overnight. Animals and other detailed methods are shown in the Supplementary Materials and Methods . All animal experiments were approved by Institutional Animal Care and Use Committee the Animal Care Committee at the University of Louisville (IACUC #12005)
3
Lentiviral Knockdown of YAP1 in Corneal Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Expression vectors encoding the YAP1 shRNA and scrambled control shRNA were purchased from Open Biosystems, Inc. Lentivirus was produced by a four-plasmid (for 3rd-generation lentiviral vectors) transfection system as described by Tiscornia et al.56 (link). Lentivirus was collected in keratinocyte serum-free medium and directly used to transduce primary corneal epithelial cells for overnight.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!