The largest database of trusted experimental protocols

8 protocols using methanol ulc ms grade

1

Decitabine Formulation Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
Decitabine was purchased from LC Laboratories® (Woburn, MA, USA). Labrafac® WL1349 (caprylic-capric acid triglycerides), Labrafil® M1944CS (oleoyl macrogol-6 glycerides), Peceol® (glycerol mono-oleates), and Transcutol® HP (THP) (highly purified diethylene glycol monoethyl ether) were kindly provided by Gattefossé (Saint-Priest, France). Lipoïd® S75-3 (soybean lecithin: 69% phosphatidylcholine and 10% phosphatidylethanolamine) and Kolliphor® HS15 (mixture of free polyethylene glycol [PEG] 660 and PEG 660 hydroxystearate) were kindly supplied by Lipoïd® and BASF, respectively (Ludwigshafen, Germany). Deionized water was obtained from a Milli-Q plus system (Merck-Millipore, Darmstadt, Germany). Sodium chloride and disodium hydrogen phosphate were purchased from Prolabo (Fontenay-Sous-Bois, France) and sucrose was purchased from Merck-Millipore. Sodium phosphate dibasic dodecahydrate, potassium dihydrogen phosphate, formic acid, ammonium acetate, Triton® X-100, Tween® 80, Span® 80, and formic acid were obtained from Sigma-Aldrich (St Quentin-Fallavier, France). Captex® 8000 (glyceryl tricaprylate) was a gift from Abitec Corp. (Saint-Quentin Fallavier, France).
Absolute ethanol, methanol ULC/MS grade, acetonitrile, and LC-MS grade water were purchased from Biosolve (Dieuze, France). Acetone was purchased from VWR (Fontenay-sous-Bois, France).
+ Open protocol
+ Expand
2

Lipid Analysis Protocol Using Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Norharmane and chloroform (≥99%) were
purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands) and used
without further purification. Methanol (ULC-MS grade), water (LC-MS
grade), ethanol (LC-MS grade), and xylene (AR grade) were purchased
from Biosolve (Valkenswaard, The Netherlands). Hematoxylin and Entellan
were purchased from Merck (Darmstadt, Germany), and eosin Y was purchased
from J.T. Baker (Center Valley, USA). Indium tin oxide (ITO) coated
glass slides were purchased from Delta Technologies (Loveland, USA).
A lipid standard of PS 18:0/18:1n-9 was purchased
from Avanti Polar Lipids (Alabaster, USA).
+ Open protocol
+ Expand
3

Fullerene Extraction and Soil Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Toluene (analytical grade), methanol (ULC/MS grade) and acetonitrile (LC-MS grade) were obtained from Biosolve B.V. (Valkenswaard, The Netherlands). Fullerenes C 60 (purity >99.9%, CAS: 99685-96-8) and C 70 (purity >99%, CAS: 115383-22-7) were purchased from Sigma-Aldrich (Steinheim, Germany). Stock solutions of the individual fullerenes were prepared in toluene at a concentration of 500 mg/L and placed on an orbital shaker (Laboshake orbital shaker, Gerhardt, K€ onigswinter, Germany) in the dark overnight. Further solutions needed for the experiments were obtained by dilution of the stock solutions and stored at 4 C in the dark and sonicated for 2 min before use (Bransonic 12, Branson, Danbury CT, United States). Quartz sand (silicon dioxide, SiO 2 ) was purchased from Sigma-Aldrich (Zwijndrecht, the Netherlands). The sandy soil consisted of a fullerenes free top-soil (top 10 cm) collected in a natural park in Oude Schulpweg, Castricum, the Netherlands (52 32 0 39.689 00 N, 4 39 0 5.623 00 E). This soil had previously been characterized by our group and had no detectable fullerenes (Carboni et al., 2016b) and 0.2% organic carbon (Carboni et al., 2016a) . The soil was placed in an oven at 65 C for one week in order to remove traces of water and then sieved with a 1.68 mm mesh.
+ Open protocol
+ Expand
4

Analytical Procedures for Authentic Dyes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aqueous solutions were prepared using deionised water (Arium 611UV, Sartorius, R = 18.2 M cm, Germany). Methanol (ULC/MS grade) was obtained from Biosolve (Valkenswaard, The Netherlands). Acetonitrile (ACN, LC-MS grade) was obtained from Avantor Performance Chemicals (Deventer, The Netherlands). Ammonium sulphate (BioXtra, ≥99%), formic acid (reagent grade, ≥95%) and tetramethylammonium-hydroxide solution (TMA, 25% in water) were obtained from Sigma-Aldrich (Darmstadt, Germany). 54 samples of authentic dyestuff from the period 1850 to 1920 were obtained from to the reference collection of the Cultural Heritage Agency of the Netherlands (RCE, Amsterdam, The Netherlands).
+ Open protocol
+ Expand
5

Lipidomic Analysis of Oxidized Phospholipids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hexane, hexadecane, Na2SO4, MES, TRIS, EGTA, ammonium formiate, 5-doxyl stearic acid, DOPC, DOPE, cardiolipin and arachidonic acid were purchased from Sigma Aldrich GmbH (Germany). ONE, HNE and HHE came from Cayman Chemicals. E. coli polar lipid and DPhPC, were purchased from Avanti polar lipids. ULC/MS grade methanol was supplied by Biosolve BV (Valkenswaard, Netherlands). Chloroform was from Merck KGaA (Darmstadt, Germany).
+ Open protocol
+ Expand
6

Solvent Preparation for Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
ULC/MS-grade water (H2O), ULC/MS-grade methanol (MeOH), LC/MS-grade ethanol (EtOH), LC/MS-grade xylene, and 99% formic acid (FA) were obtained from Biosolve (Valkenswaard, NL). Gelatin was purchased from Sigma-Aldrich. Microscope glass slides were obtained from Thermo Scientific (Braunschweig, DE).
+ Open protocol
+ Expand
7

Quantification of 6-Sulfatoxymelatonin in Biological Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
We obtained ULC-MS-grade methanol, acetonitrile, isopropanol, ammonium acetate, and formic acid from Biosolve BV (Valkenswaard, The Netherlands). Phosphate buffered saline (PBS; 0.01 M Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 0.138 M NaCl, 27 mM KCl, pH 7.4) was prepared by the Department of Pharmacy of the UMCG. 6-sulfatoxymelatonin sodium salt was purchased from Toronto Research Chemicals Inc. (Toronto, Canada), and the D 4 -labeled internal standard (IS) for 6-sulfatoxymelatonin was purchased from CacheSyn Inc. (Mississauga, Canada). Ultrapure water was produced using an in-house purification system (Merck Millipore, MA, USA).
+ Open protocol
+ Expand
8

Isoniazid and Cinnamaldehyde Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isoniazid, cinnamaldehyde, =tricaine and PVP40 were acquired from Sigma‐Aldrich and Isoniazid‐D4 internal standard from Santa Cruz Biotechnology (Santa Cruz, USA). Nanopure water was used from a PURELAB water purification system (Veolia Water Technologies B.V., Ede, The Netherlands) unless otherwise stated. ULC/MS‐grade methanol as well as ULC/MS‐grade acetonitrile, LC/MS‐grade water, and formic acid was acquired from Biosolve B.V. (Valkenswaard, The Netherlands). Difco Middlebrook 7H10 agar and 7H9 medium, OADC, and ADC were acquired from Becton Dickinson and Company (Sparks, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!