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2 protocols using anti xct slc7a11 clone d2m7a

1

Antibody Detection in Cell Lines

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Anti-KEAP1 (clone D6B12, #8047, 1:1000), anti-βactin (clone 13E5, #4970, 1:4000), anti-LKB1 (clone D60C5, #3047, 1:1000), anti-Vinculin (#4650, 1:4000), anti-TP53BP1(#4937,1:1000), anti-βcatenin (clone D10A8, #25362, 1:2000), anti-phospho-p44/42 MAPK (#9101, 1:3000), anti-phospho-MEK1/2 (clone 41G9, #9154, 1:1000 dilution), anti-xCT/SLC7A11(clone D2M7A, #12691, 1:1000) were from Cell Signaling Technology, MA. Anti-NRAS antibody (#ab77392) was from Abcam, MA. Erastin (#17754) was from Cayman Chemical. Sotorasib (#HY-114277) used ex vivo was from MedChemExpress.
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2

Investigating Cellular Signaling Pathways

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Anti-KEAP1 (clone D6B12, #8047, 1:1,000), anti–β-actin (clone 13E5, #4970, 1:4,000), anti-LKB1 (clone D60C5, #3047, 1:1,000), anti-Vinculin (#4650, 1:4,000), anti-TP53BP1(#4937, 1:1,000), anti-βcatenin (clone D10A8, #25362, 1:2,000), anti-phospho-p44/42 MAPK (#9101, 1:3,000), anti-phospho-MEK1/2 (clone 41G9, #9154, 1:1,000 dilution), anti-xCT/SLC7A11 (clone D2M7A, #12691, 1:1,000) were from Cell Signaling Technology, MA. Anti-NRAS antibody (#ab77392) was from Abcam, MA. Erastin (#17754) was from Cayman Chemical. Sotorasib (#HY-114277) used ex vivo was from MedChemExpress.
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