Rabbit ezh2

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit EZH2 is a primary antibody product offered by Cell Signaling Technology. It is a purified rabbit monoclonal antibody that recognizes the enhancer of zeste homolog 2 (EZH2) protein, which is a histone methyltransferase that plays a key role in the epigenetic regulation of gene expression.

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Lab products found in correlation

Close spelling variants of this product

Rabbit anti-EZH2 (20 citations) Rabbit anti-human EZH2 (3 citations) Rabbit anti-human EZH2 antibody (2 citations) Anti-EZH2 or anti-rabbit IgG antibodies (2 citations)

3 protocols using rabbit ezh2

Comprehensive Protein Immunoblotting and IHC Analysis

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Based on the appropriate experimental time points, cells were lysed by standard procedure in lysis buffer containing a protease inhibitor cocktail. Protein lysates (50 µg) were resolved on SDS-PAGE gels followed by immunoblot detection and visualization with ECL kit (PerkinElmer). Antibodies and concentrations used for immunoblots are as follows: BCLAF1 (Bethyl laboratories #A300-608; 1:1000), DNMT1 (abcam #ab1353; 1:1000), Cell Signaling antibodies used at 1:1000 dilution- rabbit RSK (#9355), rabbit p-RSK, rabbit S6K1 (#9202), rabbit p-S6K1 (#9208), rabbit β-tubulin (#2146), rabbit RSK2 (#9340), rabbit ERK1/2 (#4695), rabbit p-ERK1/2 (#9101), rabbit p-AKT(S473) (#9271), rabbit AKT (#9272), rabbit PDGFRα (#3164), rabbit EGFR (#4267), rabbit p-EGFR (#3777), rabbit EZH2 (#5246), rabbit SUZ12 (#3737), rabbit p-STAT3 (#9145) and mouse STAT3 (#9139). For IHC analysis, brain glioblastoma and normal tissue array (40 cases/80 cores) was purchased from Biomax (GL806). The paraffin embedded tissue array processing for IHC analysis was performed as described earlier (1 (link)). Antibodies and the concentrations used for IHC and IF are as follows: rabbit S6K1 (1:50, Cell Signaling), rabbit RSK2 (1:100, Cell Signaling).

Mathew L.K., Huangyang P., Mucaj V., Lee S.S., Skuli N., Eisinger-Mathason T.S., Biju K., Li B., Venneti S., Lal P., Lathia J.D., Rich J.N., Keith B, & Simon M.C. (2015). Feedback circuitry between miR-218 repression and RTK activation in Glioblastoma. Science signaling, 8(375), ra42.

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Antibody Selection for Western Blot and IHC

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Species and dilutions of primary antibodies used for Western Blotting and immunohistochemistry experiments were as follows: rabbit anti-CDK1 (1:1′000; Santa Cruz Biotechnology, Santa Cruz, USA), rabbit PRC1 (1:1000, Protein Tech Group, Chicago, USA), rabbit EZH2 (1:1000, Cell Signaling, Danvers), rabbit Ki67 (Abcam, Cambridge, UK), Bax (Santa-Cruz sc-493), Bcl-2 (Cell Signaling, 2876), Bcl-Xl (Cell Signaling, 2764), Bim (Cell Signaling, 2819), rabbit ERK (1:1000, Santa Cruz Biotechnology, Santa Cruz, USA), mouse P-ERK (1:1000, Santa Cruz Biotechnology, Santa Cruz, USA), rabbit CCND1 (1:1000, Santa Cruz Biotechnology, Santa Cruz, USA), mouse anti-AKT (Santa Cruz Biotechnologies Inc., Dallas, TE, USA; sc-5298), rabbit anti-P-AKT (Cell Signaling; #4060), and mouse Actin (1:1000; Sigma, St. Louis, USA).
The secondary antibodies used for western blotting and immunohistochemistry experiments were goat anti-rabbit HRP or goat anti-mouse HRP (Amersham Biosciences, Otelfingen, Switzerland) and Alexa Fluor 594 goat anti-rabbit (Molecular Probes, Invitrogen Inc., Eugene, OR, USA).

Allaman-Pillet N., Oberson A, & Schorderet D.F. (2017). Bigh3 silencing increases retinoblastoma tumor growth in the murine SV40-TAg-Rb model. Oncotarget, 8(9), 15490-15506.

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Antibody Profiling for Viral Infection

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The following antibodies were used for immunofluorescence, Co-IP, and Western blotting studies: rat polyclonal anti-HHV8 or anti-LANA [LN53] (Abcam, ab4103), rabbit anti-Coilin (Cell Signaling technology,14168S), rabbit anti-DAXX (Sigma, D7810), rabbit anti-PML (Bethyl, A301167A), mouse anti-ORF50 (provided by Erle Robertson, UPENN), mouse anti-ORF45 (provided by Yan Yuan, UPENN), mouse anti-EBV (BIO-RAD, 4260–0906), anti-RAD21 (Abcam ab992), rabbit polyclonal anti-PARP1 (Enzo ALX-210-302-R100), rabbit EZH2 (Cell Signaling, 4905), rabbit H3K27me3 (Active Motif, 39155), rabbit IgG (Santa Cruz Biotechnology), AlexaFluor594 or AlexaFluor488 (Invitrogen), and mouse anti-Actin-HRP (Sigma, A23852).

Vladimirova O., De Leo A., Deng Z., Wiedmer A., Hayden J, & Lieberman P.M. (2021). Phase separation and DAXX redistribution contribute to LANA nuclear body and KSHV genome dynamics during latency and reactivation. PLoS Pathogens, 17(1), e1009231.

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