Ab137994

Cardiomyocytes were seeded in 96‐well plates. When the cells reached 70% confluence, the cell supernatant was harvested. According to the specifications of ELISA kits (ab137994, Abcam Inc), the concentration of interleukin 1β (IL‐1β), interleukin‐6 (IL‐6), interleukin‐10 (IL‐10) and tumour necrosis factor α (TNF‐α) in the supernatant was calculated through a linear regression equation of the standard curve.

Commercial enzyme-linked immunosorbent assay [ELISA] kits from Abcam (Paris, France) were used to measure plasma (PFP) concentrations of fibrinogen (ref: ab108844) or TAT complexes (ref: ab137994). TF in aorta and kidney was measure by the commercial ELISA kit according to manufacturer's instructions (Abcam, ref: ab214091).

Thrombin–Antithrombin (TAT) complexes were measured in blood plasma by ELISA using a commercial kit (cat. #ab137994, Abcam). The assay was performed according to manufacturer's instructions. The chromogen substrate was incubated for 20 min and the absorbance was read at 450 nm using VersaMax™ microplate reader. The TAT ELISA was performed on P10 plasma (6 Jag1^+/+ and 6 Jag1^Ndr/Ndr mice). Fibrinogen was measured in P10 serum (6 Jag1^+/+ and 6 Jag1^Ndr/Ndr mice) using a commercial kit (cat. #ab213478, Abcam), according to manufacturer's instructions. The chromogen substrate was incubated for 15 min and the absorbance was read at 450 nm using VersaMax™ microplate reader. INR was measured in a drop of fresh whole blood (6 Jag1^+/+ and 6 Jag1^Ndr/Ndr mice at P10) with a commercially available point of care coagumeter (CoaguChek® XS, Roche). Tail bleeding time was assessed in 6 Jag1^+/+ and 6 Jag1^Ndr/Ndr mice at P10 in anesthetized (Isoflurane inhalation) pups by severing the tip of the tail (2 mm from the tip) and gently wiping the tail on a tissue paper without squeezing the tail. The time was recorded from the moment the tail was cut. The tail was wiped every 20 s to observe the bleeding. The bleeding was considered stopped when no bleeding reappeared after 1 min.