Log In Sign up
The largest database of trusted experimental protocols

The HTB-55 is a benchtop incubator designed for temperature-controlled storage of samples. It features a digital temperature display and adjustable temperature controls to maintain a consistent environment for your laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

L glutamax, by Thermo Fisher Scientific (2 mentions) 293t ace2 cells, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Vero e6 cell, by American Type Culture Collection (2 mentions) Sars cov 2 isolate usa wa1 2020, by BEI Resources (2 mentions) Vero e6 cells, by BEI Resources (2 mentions) Penicillin streptomycin, by Corning (2 mentions)

2 protocols using htb 55

1

SARS-CoV-2 Propagation and Titration

Check if the same lab product or an alternative is used in the 5 most similar protocols
For studies at the Gladstone Institutes, Calu-3 cells, a human lung epithelial cell line (American Type Culture Collection, ATCC HTB-55), were cultured in advanced MEM supplemented with 2.5% fetal bovine serum (FBS) (Gibco, Life Technologies), 1% l-GlutaMax (ThermoFisher), and 1% penicillin/streptomycin (Corning) at 37 °C and 5% CO2. SARS-CoV-2 Isolate USA-WA1/2020 was purchased from BEI Resources and propagated and titered in Vero E6 cells. For studies carried out at Mount Sinai, SARS-CoV-2 was propagated in Vero E6 cells (ATCC CRL-1586) and 293T-ACE2 cells (ATCC CRL-3216).
Le B.L., Andreoletti G., Oskotsky T., Vallejo-Gracia A., Rosales R., Yu K., Kosti I., Leon K.E., Bunis D.G., Li C., Kumar G.R., White K.M., García-Sastre A., Ott M, & Sirota M. (2021). Transcriptomics-based drug repositioning pipeline identifies therapeutic candidates for COVID-19. Scientific Reports, 11, 12310.
+ Open protocol
+ Expand
2

SARS-CoV-2 Propagation in Lung Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For studies at the Gladstone Institutes, Calu-3 cells, a human lung epithelial cell line (American Type Culture Collection, ATCC HTB-55), were cultured in advanced MEM supplemented with 2.5% fetal bovine serum (FBS) (Gibco, Life Technologies), 1% L-GlutaMax (ThermoFisher), and 1% penicillin/streptomycin (Corning) at 37°C and 5% CO2. SARS-CoV-2 Isolate USA-WA1/2020 was purchased from BEI Resources and propagated and titered in Vero E6 cells. For studies carried out at Mount Sinai, SARS-CoV-2 was propagated in Vero E6 cells (ATCC CRL-1586) and 293T-ACE2 cells (ATCC CRL-3216).
Le B.L., Andreoletti G., Oskotsky T., Vallejo-Gracia A., Rosales R., Yu K., Kosti I., Leon K.E., Bunis D.G., Li C., Kumar G.R., White K.M., García-Sastre A., Ott M, & Sirota M. (2021). Transcriptomics-based drug repositioning pipeline identifies therapeutic candidates for COVID-19. Research Square.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!