Blood samples were collected upon each patient's hospital arrival in the morning in a fasting state at enrollment and at the end of the study. The blood samples were centrifuged at 3000 g for 15 min at room temperature. Plasma/serum samples after separation were stored at 4°C in refrigerated containers and sent to a commercial laboratory (SRL) within 24 h. All assays were performed within 24 h of arrival of the samples at this single laboratory center. Using the stored serum samples, Hs‐cTnT was measured using a highly sensitive assay on an automated platform (Elecsys‐2010 Troponin Ths STAT; Roche Diagnosis), with a lower detection limit of 0.003 ng/ml and a reported 99th percentile value in apparently healthy individuals of 0.014 ng/ml.32 Serum NT‐pro BNP levels were determined using a chemiluminescence immunoassay kit (Roche Diagnostics). The lower limit of detection of NT‐pro BNP is 5 pg/ml. The cutoff point (125 pg/ml) for NT‐pro BNP followed the diagnostic criteria for suspected heart failure in the European Society of Cardiology heart failure guidelines.33 The intra/inter‐coefficients of variation were 2.02%/3.02% for Hs‐cTnT and 1.93%/3.13% for NT‐pro BNP.
Chemiluminescence immunoassay kit
Manufactured by Roche
Sourced in Switzerland
The Chemiluminescence Immunoassay Kit is a laboratory equipment used for the detection and quantification of specific analytes in a sample. It utilizes the principle of chemiluminescence, where a chemical reaction produces light that can be measured to determine the concentration of the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using chemiluminescence immunoassay kit
1
Biomarker Measurement in Fasting Blood Samples
2
Mouse Serum Hormone Levels Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse serum follicle-stimulating hormone (FSH), estradiol (E2), and progesterone (P4) levels were measured using a chemiluminescence immunoassay kit (Roche Diagnostics, Basel, Switzerland) on a Roche Diagnostics Cobas 6000 analyzer.
3
Measuring Serum Biomarkers in Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fasting venous blood was drawn from all patients within 24 h of admission, centrifuged at 3000 r/min for 10 min, and the serum was separated. Serum BNP levels were determined using a chemiluminescence immunoassay kit (Roche Diagnostics). Serum PTX3 and VEGF levels were measured by enzyme‐linked immunosorbent assay (ELISA) kits (Boster).
4
Measuring Mouse Serum AMH
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was taken from the inner canthus vein of mice, and serum was collected. Mouse serum AMH level was measured for each group using a chemiluminescence immunoassay kit (Roche Diagnostics, Basel, Switzerland) according to the manufacturer's introduction on a Roche Diagnostics Cobas 6000 analyzer.
5
Fasting Lipid and Glucose Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting serum concentrations of triglyceride (TG), total choleste rol (TC), high-density lipoprotein (HDL) cholesterol, creatinine (Cr), AST and ALT, and the fasting plasma glucose (FPG) concentrations were measured using enzymatic methods, and the PLT was measured using the sheath flow method (Falco Biosystems Co., Ltd., Tokyo, Japan). Serum NT-proBNP levels were determined using a chemiluminescence immunoassay kit (Roche Diagnostics, Mannheim, Germany). All blood samples were obtained in the morning after the participants had fasted overnight. The FIB-4 score was calculated using the following formula: age ([yr] × AST [U/L])/(PLT [109/L]) × (ALT [U/L])1/2) [19 (link)].
6
Fasting Blood Biomarkers Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting serum concentrations of triglyceride, total cholesterol, high‐density lipoprotein cholesterol, and creatinine, fasting plasma glucose, and HbA1c concentrations were measured using enzymatic methods (Falco Biosystems Co., Ltd., Tokyo, Japan). Serum NT‐proBNP levels were determined using a chemiluminescence immunoassay kit (Roche Diagnostics, Mannheim, Germany).21 (The sensitivity was 88%, and specificity was 92%; the inter‐observer and intra‐observer coefficients of variation were 1.8% and 1.6%, respectively.) All the blood samples were obtained in the morning after the patients had fasted overnight.
7
Serum CA19-9 Levels in Pancreatic Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum CA19-9 level was detected using Roche chemiluminescence immunoassay kit. Preoperative serum CA19-9 was detected just before the surgery so as to evade the effect of hyperbilirubinemia on CA19-9 level. Postoperative serum CA19-9 level was measured between 1 and 3 months after surgery. Preoperative biliary drainage was conducted on patients with obstructive jaundice using endoscopic retrograde biliary drainage or percutaneous transhepatic biliary drainage. Patients with preoperative total bilirubin levels > 34.2 umol/l after biliary drainage were excluded. Both preoperative and postoperative CA19-9 levels were divided as ≤ 37 U/ml vs. > 37 U/ml, ≤ 100 U/ml vs. > 100 U/ml, ≤ 150 U/ml vs. > 150 U/ml, ≤ 200 U/ml vs. > 200 U/ml, ≤ 400 U/ml vs. > 400 U/ml, ≤ 1000 U/ml vs. > 1000 U/ml. These cut-off values were selected in the light of many previous studies and the distribution of CA19-9 levels in our current study [15 (link), 16 (link), 22 (link), 27 (link), 28 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!