Pd l1 ig

A total of 50 female CBA/J, 20 male DBA/2 and 5 male BALB/c mice (age, 8–10 weeks; weight, 12–15 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. The mice were maintained under controlled conditions at 19–23°C with 12-h light/dark cycles and 40–60% humidity, with free access to drinking water and food. Animal experiments were approved by the Institutional Animal Care and Use Committee of Kunming Medical University.
The mice were divided into the following five groups: the normal group (10 CBA/J mice), the spontaneous abortion group (10 CBA/J mice), the PD-L1 Ig group (0.1 mg/kg PD-L1 Ig; 10 CBA/J mice), the CD40L mAb group (0.2 mg/kg anti-CD40L mAbs; 10 CBA/J mice) and the PD-L1 Ig + CD40L mAb group (0.1 mg/kg PD-L1 Ig and 0.2 mg/kg anti-CD40L mAbs; 10 CBA/J mice). The normal group was mated with male BALB/c mice (n=5). The spontaneous abortion, PD-L1 Ig, CD40L mAb and PD-L1 Ig + CD40 mAb groups were mated with male DBA/J mice (n=5/group). The day the vaginal plug was observed was recorded as day 0 of gestation. PD-L1 Ig and/or anti-CD40L mAbs were injected intraperitoneally into CBA/J female mice at days 4, 6, 8, 10 and 12 of gestation. At day 14 of gestation, the CBA/J mice were euthanized with an overdose of sodium pentobarbital (150 mg/kg; intraperitoneally). PD-L1 Ig and anti-CD40L mAbs were purchased from R&D Systems.