ShRNA lentiviral particle delivery system was used to generate IGF2BP3 shRNA and IGF2BP3‐silenced tumour cell lines according to the manufacturer's instructions (Sigma‐Aldrich). The lentiviral particles were purchased from Sigma‐Aldrich. After selection under puromycin (1 μg/ml), the knocking down effect in the drug‐resistant cells was evaluated by Western blot.
Shrna lentiviral particle delivery system
Manufactured by Merck Group
Sourced in United States
The ShRNA lentiviral particle delivery system is a laboratory tool used for the stable introduction of short hairpin RNA (shRNA) sequences into target cells. It enables the knockdown or silencing of specific genes in order to study their functions. The system utilizes lentiviral particles to efficiently transduce and integrate the shRNA construct into the host cell genome.
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Lab products found in correlation
3 protocols using shrna lentiviral particle delivery system
1
Lentiviral Knockdown of IGF2BP3
2
STAT1 Silencing via Lentiviral shRNA
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ShRNA lentiviral particle delivery system was used to generate STAT1 siRNA, and STAT1-silenced tumor cell lines were done according to the manufacturer's instructions (Sigma-Aldrich, Saint Louis, MO, USA). The lentiviral particles were purchased from Sigma (Sigma-Aldrich, Saint Louis, MO, USA). After selection under puromycin (1μg/ml), the knocking down effect in the drug resistant cells was evaluated by western blot.
3
Silencing of EMMPRIN, STAT3, and HIF-1α in Tumor Cells
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shRNA lentiviral particle delivery system was used to generate EMMPRIN, STAT3, and HIF-1α-silenced tumor cell lines according to the manufacturer’s instructions (Sigma-Aldrich, Saint Louis, MO, USA). The lentiviral particles were purchased from Sigma (Sigma-Aldrich, Saint Louis, MO, USA). After selection under puromycin (1 μg/ml), the knocking down effect in the drug resistant cells was evaluated by western blot.
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