Cell dissociation reagent

Fetal bovine serum (FBS), Cell Dissociation Reagent, medium LHC-9 and Dulbecco’s modified Eagle medium (DMEM) were provided by Invitrogen (Carlsbad, CA). The antibody against VECTOR ImmPRESS Anti-Goat Ig was provided by Santa Cruz Biotechnology (Santa Cruz, CA). Protein Block solution and DAB substrate were provided by Abcam (Cambridge, MA). Antibodies against vimentin, E-cadherin, N-cadherin and TCTP were purchased from Cell Signaling (Boston, MA). ECL reagent kit was purchased from GE Healthcare Life Sciences (Piscataway, NJ). NNK was purchased from Chemsyn Science Laboratories (Lenexa, KS). 10 µM NNK was used in the experiments [20 (link)]. PM_2.5 was collected at Kowloon Tong of Hong Kong and 5 µg/ml PM_2.5 was used in the experiments as details described in our previous study [21 (link)].

Fetal bovine serum (FBS), Cell Dissociation Reagent, Dulbecco’s modified Eagle medium (DMEM), LHC-9, and RPMI medium 1640 were provided by Invitrogen (Carlsbad, CA). Cancer Stem Cell Media Premium™ was purchased from ProMab Biotechnologies (Richmond, CA). The antibody against VECTOR ImmPRESS Anti-Goat Ig was provided by Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against CD133 were provided by Proteintech (#18470–1-AP, Rosemont, IL). Antibodies against 15-LOX2 were provided by lifespan (#LS-B1588). Antibodies against ALDH1A1 (#ab23375), 15-LOX1 (#ab80221), Protein Block solution and DAB substrate were provided by Abcam (Cambridge, MA). Antibodies against Bcl-2 (##4223), Bax (#14796S), pGSK3β (#5558 s), GSK3β (#12456 s), Nanog (#4903), TCF4 (#2569 s) and β-catenin (#8480 s) were purchased from Cell Signaling (Boston, MA). ECL reagent kit was purchased from GE Healthcare Life Sciences (Piscataway, NJ). 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) was purchased from Sigma (St, Louis, MO). Ultra-low-adhesion 6 well plates and 10 mm plates were purchased from Corning (Tewksbury, MA).