Human PBL were treated with PGE2 for 3 d. After this, cells were harvested, washed in PBS buffer, and then stained with the following monoclonal antibodies (mAbs): CD4-PC7, CCR5-PE, CXCR4-FITC, CD69-PC5, CD38-FITC (all from BD Biosciences). After incubation with the mAbs for 30 min at 4°C in the dark, the cells were washed and fixed in 2% paraformaldehyde solution for flow cytometric analysis using a FACScalibur cytometer (BD Biosciences, CA). Live cells were gated according to their forward- and side-scatter profiles. These experiments were repeated at least three times using cells from different donors.
Cxcr4 fitc
Manufactured by BD
CXCR4-FITC is a fluorescently labeled antibody that binds to the CXCR4 receptor. It is used for the detection and analysis of CXCR4-expressing cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Ccr5 pe, by BD (1 mentions)
Cd4 pc7, by BD (1 mentions)
Cd69 pc5, by BD (1 mentions)
Cd38 fitc, by BD (1 mentions)
Facscalibur cytometer, by BD (1 mentions)
Cd4 pe, by BD (1 mentions)
Lc3b ab, by Cell Signaling Technology (1 mentions)
Sca 1 fitc, by BioLegend (1 mentions)
Cd34 pe, by BD (1 mentions)
C kit apc, by BD (1 mentions)
Ly6g apc, by BD (1 mentions)
Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Rockland Immunochemicals (1 mentions)
Sca1 apc, by BioLegend (1 mentions)
3 protocols using cxcr4 fitc
1
Phenotypic Characterization of PGE2-Treated Human PBLs
2
Characterization of CD4+ T Cell Markers
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This assay was performed according to the guidelines of BD Pharmingen and approved by Shanghai Changhai Hospital, primary CD4+ T cells isolated from blood of healthy donors were stimulated with different LRAs for 72 hours and then stained with CD4-PE, CXCR4-FITC and CCR5-FITC antibodies (BD Biosciences) to subject to flow cytometry analysis.
3
Antibodies for Western Blot, Immunofluorescence, and Flow Cytometry
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The following primary antibodies were used for western blot and immunofluorescence imaging: DNM2 Ab, Santa Cruz (#sc-6400, #C-18; Dallas, TX); and CXCR4 Ab, Santa Cruz (#sc-9046, H-118). The following secondary antibodies were used: horseradish peroxidase–conjugated secondary antibodies were either from Rockland (Limerick, PA) or from Santa Cruz; Alexa Fluor secondary antibodies were from Thermo Fisher Scientific. The following antibodies were used for immunohistochemistry: Ly6G Ab, Cell Signaling (#87048; Danvers, MA), BCL-6 Ab, Abcam (#ab243150; Waltham, MA), and LC3B Ab, Cell Signaling (#83506). The following antibodies were used for flow cytometry experiments: CXCR4-FITC, BD Biosciences (#561735; Franklin Lake, NJ), and Ly6G-APC, BD Biosciences (#560599), c-kit APC, BD Pharmingen (Franklin Lake, NJ, #553356), IL7R BV421 (#135023; Biolegend), CD34-PE (#551387; BD Pharmingen), CD16/32-PE-Cy7 (#25-0161-82; ThermoFisher), Sca-1-FITC (#122505; Biolegend). In Cyto-ID autophagy experiments, Sca-1 APC (#108112; Biolegend) was used.
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